Lange L, Fritzsch T, Hilmann J, Kubowicz G, Mützel W
Arzneimittelforschung. 1986 Jul;36(7):1034-6.
The right-ventricular echocontrast provided by 5 different concentrations of the new standardized sonographic contrast agent SH U 454 (100, 150, 200, 300 and 400 mg microparticles/ml), which consists of pure galactose microparticles, was examined in 3 anesthetized female beagle dogs (8.6-9.3 kg) in order to assess the influence of the period of time which elapsed between preparation of the suspension and its injection. Each animal was given 2 ml of an SH U 454 concentration by intravenous injection at a speed of approximately 2 ml/s immediately after preparation of the suspension and 1, 2, 3 and 5 min thereafter. Evaluation of the intensity of contrast was performed blind by two investigators working independently and using a visual scoring system (ratings 0-5) and also by a videodensitometer. The videodensitometer provided a quantitative evaluation of the maximum intensity of contrast, the duration of contrast and the area under the videodensitometer curve. The intensity of the contrast of the prepared suspension subsided at a constant rate over a period of 5 min for all the concentrations tested. However, the difference between the starting value t0min and the terminal value t5min was small. On the whole, very little difference was found over the 5-min period in the intensity of the contrast provided by any of the SH U 454 concentrations examined. Thus, for the injection the examiner has a timeframe of at least 5 min after preparation of the suspension.
由5种不同浓度（100、150、200、300和400毫克微粒/毫升）的新型标准化超声造影剂SH U 454（由纯半乳糖微粒组成）提供的右心室超声造影，在3只麻醉的雌性比格犬（8.6 - 9.3千克）身上进行了检测，以评估混悬液制备与注射之间经过的时间的影响。在混悬液制备后立即以及此后1、2、3和5分钟，以大约2毫升/秒的速度通过静脉注射给每只动物注射2毫升不同浓度的SH U 454。由两名独立工作的研究人员使用视觉评分系统（0 - 5级）并通过视频密度计对造影强度进行盲法评估。视频密度计对造影的最大强度、造影持续时间和视频密度计曲线下面积进行了定量评估。对于所有测试浓度，制备好的混悬液的造影强度在5分钟内以恒定速率下降。然而，起始值t0分钟与终末值t5分钟之间的差异很小。总体而言，在所检测的任何SH U 454浓度提供的造影强度在5分钟内差异非常小。因此，对于注射而言，检查人员在混悬液制备后至少有5分钟的时间范围。
