Rao Huma, Rao Iqra, Saeed Laiba, Aati Hanan Y, Aati Sultan, Zeeshan Muhammad, Ur Rehman Khan Kashif
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Punjab, Pakistan.
King Edward Medical University, Lahore, Punjab, Pakistan.
Saudi J Biol Sci. 2023 Oct;30(10):103783. doi: 10.1016/j.sjbs.2023.103783. Epub 2023 Aug 22.
Plants have always been the prime focus in medicine industries due to their enormous ethnobotanical uses and multitude of biological and therapeutic properties. In the current study, preliminary phytochemical composition, Total phenolic content (TPC), and total flavonoid content (TFC) with the antioxidant and antibacterial activity of hydroalcoholic extract and n-hexane, chloroform and n-butanol fractions of five selected medicinal plants [ (L.) Pers., L., (Wall. ex Cambess.) Hook, Thunb., and (Lam.) Pers.] from Pakistan, have been evaluated. TPC and TFC were determined by Folin-Ciocalteu's and AlCl methods respectively. The antioxidant activity was performed by DPPH, ABTS, FRAP, and CUPRAC while the antibacterial potential of these plants was determined by agar well diffusion assay. . (Lam.) Pers. exhibited the highest TPC (695 ± 13.2 mg.GA.Eq.gDE ± SD) in n-butanol fraction and the highest TFC in its chloroform faction (615 ± 6.31 mg Q.Eq.g DE ± SD). The n-butanol fraction and hydroalcoholic extract of Thunb. exhibited strong antioxidant potential by DPPH and CUPRAC assays respectively, whereas . (Lam.) Pers. n-butanol fraction exhibited the strongest reducing potential. The hydroalcoholic extract of all tested plants exhibited significant antibacterial activity against tested bacterial strains with (12-18 mm). Conclusively, . (Lam.) Pers. (Family: Crassulaceae) and . Thunb. (Family: Iridaceae) exhibited the highest antioxidant and antibacterial potential. They can be explored for the isolation of phytoconstituents responsible for this potential and serve as a lead for the production of new natural antioxidants and antibacterial agents that can be used to cure various diseases.
由于植物具有巨大的民族植物学用途以及众多的生物学和治疗特性，它们一直是医药行业的主要关注点。在当前的研究中，对五种选自巴基斯坦的药用植物[（L.）Pers.、L.、（Wall. ex Cambess.）Hook、Thunb.和（Lam.）Pers.]的水醇提取物以及正己烷、氯仿和正丁醇馏分的初步植物化学成分、总酚含量（TPC）、总黄酮含量（TFC）以及抗氧化和抗菌活性进行了评估。TPC和TFC分别通过福林-西奥尔特法和氯化铝法测定。抗氧化活性通过DPPH、ABTS、FRAP和CUPRAC法进行检测，而这些植物的抗菌潜力则通过琼脂扩散法测定。（Lam.）Pers.在正丁醇馏分中表现出最高的TPC（695±13.2 mg.GA.Eq.gDE±SD），在其氯仿馏分中表现出最高的TFC（615±6.31 mg Q.Eq.g DE±SD）。Thunb.的正丁醇馏分和水醇提取物分别通过DPPH和CUPRAC法表现出较强的抗氧化潜力，而（Lam.）Pers.的正丁醇馏分表现出最强的还原潜力。所有受试植物的水醇提取物对受试细菌菌株均表现出显著的抗菌活性（抑菌圈为12 - 18 mm）。总之，（Lam.）Pers.（景天科）和Thunb.（鸢尾科）表现出最高的抗氧化和抗菌潜力。可以对它们进行研究，以分离出具有这种潜力的植物成分，并作为生产可用于治疗各种疾病的新型天然抗氧化剂和抗菌剂的先导。
