Mack S R, Samuels S, Vanderberg J P
J Parasitol. 1979 Feb;65(1):130-6.
Determinations were made of free amino acids in hemolymph collected from adult female Anopheles stephensi mosquitoes. The hemolymph first was fractionated by extraction and precipitation procedures, after which qualitative determinations of free amino acids were made by high voltage thin layer electrophoresis, and thin layer chromatography. Subsequent quantitative determinations were made with an automatic amino acid analyzer. The concentration of total free amino acids in the hemolymph rose 60--70% after the mosquito took a blood meal, and remained relatively constant thereafter. When mosquitoes took a blood meal infected with the rodent malaria parasite Plasmodium berghei, the rise in total free amino acids was only 15--25%. The chief differences that occurred with individual free amino acids was that infected mosquitoes had greater increases in arginine, greater decreases in valine and histidine, and a total loss of detectable methionine.
对成年雌性斯氏按蚊采集的血淋巴中的游离氨基酸进行了测定。血淋巴首先通过提取和沉淀程序进行分级分离，之后通过高压薄层电泳和薄层色谱对游离氨基酸进行定性测定。随后使用自动氨基酸分析仪进行定量测定。蚊子吸食血液后，血淋巴中总游离氨基酸的浓度上升了60% - 70%，此后保持相对稳定。当蚊子吸食感染了啮齿类疟原虫伯氏疟原虫的血液时，总游离氨基酸的上升仅为15% - 25%。单个游离氨基酸出现的主要差异在于，受感染的蚊子精氨酸增加幅度更大，缬氨酸和组氨酸减少幅度更大，且可检测到的蛋氨酸完全消失。
