Heidrich H G, Danforth H D, Leef J L, Beaudoin R L
J Parasitol. 1983 Apr;69(2):360-7.
Sporozoites of the rodent malaria, Plasmodium berghei, were obtained from infected Anopheles stephensi by grinding mosquitoes, prepurifying the material in a discontinuous Hypaque gradient and further purifying by means of continuous free-flow electrophoresis. Bacteria, debris, mitochondria, mitoplasts, and other contaminants were removed in the electric field. The isolated sporozoites were morphologically intact and were positive in indirect immunofluorescence assay. They were infective to mice prior to and following free-flow electrophoretic separation. The surface of the sporozoites exhibited a polysaccharide-rich layer. The predominant surface protein labelled after surface iodination had a molecular weight between 42,000 and 46,000 daltons.
通过研磨感染伯氏疟原虫的斯氏按蚊，在不连续的异泛影葡胺梯度中对材料进行预纯化，并通过连续自由流动电泳进一步纯化，从而获得啮齿动物疟疾伯氏疟原虫的子孢子。在电场中去除了细菌、碎片、线粒体、线粒体质和其他污染物。分离出的子孢子形态完整，间接免疫荧光试验呈阳性。在自由流动电泳分离之前和之后，它们对小鼠均具有感染性。子孢子表面有一层富含多糖的层。表面碘化后标记的主要表面蛋白的分子量在42,000至46,000道尔顿之间。
