弓蛔虫酶联免疫吸附测定吸光度值与弓蛔虫酶联免疫吸附测定滴度之间的关系：一项对比研究。

The relationship between toxocara ELISA absorbance values and toxocara ELISA titres: a comparative study.

作者信息

Clemett R S, Hidajat R R, Allardyce R A, Stewart A C

出版信息

Aust N Z J Ophthalmol. 1986 Aug;14(3):199-203. doi: 10.1111/j.1442-9071.1986.tb00036.x.

DOI:10.1111/j.1442-9071.1986.tb00036.x

Abstract

Enzyme-linked immunosorbent assays (ELISAs) may be made by a variety of techniques at one or several serum dilutions which is very confusing for clinicians. This study compares the ELISA results from two centres on sera from 12 patients investigated for ocular toxocariasis by two common ELISA methods--the absorbance and titration techniques. With one exception, ELISA results by the two methods correlated well. For reasons outlined, the absorbance ELISA method is likely to remain the preferred method in New Zealand and Australia. Attention is drawn to the implications of the serum response to Toxocara canis larvae, and the need to evaluate ELISA results against clinical findings is emphasized.

摘要

酶联免疫吸附测定（ELISA）可以通过多种技术在一种或几种血清稀释度下进行，这对临床医生来说非常困惑。本研究比较了两个中心采用两种常见ELISA方法（吸光度法和滴定法）对12例因眼部弓蛔虫病接受调查的患者血清进行ELISA检测的结果。除了一个例外，两种方法的ELISA结果相关性良好。出于所述原因，吸光度ELISA方法可能仍将是新西兰和澳大利亚的首选方法。文中提请注意血清对犬弓蛔虫幼虫反应的影响，并强调需要根据临床发现评估ELISA结果。