Glickman L T, Grieve R B, Lauria S S, Jones D L
J Clin Pathol. 1985 Jan;38(1):103-7. doi: 10.1136/jcp.38.1.103.
This study was designed to compare the sensitivity and specificity of enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of ocular toxocariasis using Toxocara canis embryonated egg antigen (TEE) and toxocara excretory-secretory or exoantigen (TEX) produced in vitro. TEE and TEX ELISA were comparably sensitive, but TEX ELISA was better able to discriminate between serum samples from patients with ocular toxocariasis and those from patients with retinoblastoma. In addition, preabsorption of sera with Ascaris suum embryonated egg antigen seemed to be essential to prevent false positive results with TEE ELISA but was not so critical for TEX-ELISA. Further studies are still required to standardise TEX for serodiagnosis.
本研究旨在比较酶联免疫吸附测定（ELISA）使用犬弓首蛔虫感染性虫卵抗原（TEE）和体外产生的犬弓首蛔虫排泄分泌或外抗原（TEX）对眼弓蛔虫病进行血清学诊断的敏感性和特异性。TEE ELISA和TEX ELISA的敏感性相当，但TEX ELISA在区分眼弓蛔虫病患者和视网膜母细胞瘤患者的血清样本方面表现更佳。此外，用猪蛔虫感染性虫卵抗原对血清进行预吸附似乎是防止TEE ELISA出现假阳性结果的关键，但对TEX ELISA而言并非如此关键。仍需进一步研究以标准化用于血清学诊断的TEX。
