Department of Gynecology, Zhaoqing First People's Hospital, Zhaoqing, China.
Medicine (Baltimore). 2023 Sep 8;102(36):e35135. doi: 10.1097/MD.0000000000035135.
To investigate the molecular targets and mechanisms of compound kushen injection (CKI) in the prevention and treatment of cervical cancer based on network pharmacology and transcriptomics.
In this study, we used network pharmacology methods to screen for effective compounds, integrated the results of network pharmacology and RNA-seq to comprehensively screen and predict target genes, analyze the biological functions and signaling pathways of target genes, and construct a PPI network to screen for hub genes. The results were further verified by biological experiments, molecular docking, RT-PCR, and western blot analysis.
The results showed that the hub genes CXCL2, anti-vascular endothelial growth factor, hexokinase 2 are therapeutic targets of CKI for the treatment of Cervical Cancer. These targets were significantly enriched in pathways mainly including pathways in cancer, cell cycle, MAPK signaling pathways, etc. In vitro cell experiments showed that CKI could effectively inhibit the proliferation of cancer cells, promote apoptosis, and induce cell cycle arrest. RT-PCR and western blot experiments showed that the expression of hub genes was significantly decreased. The compounds have good binding activity to hub genes.
CKI, based on its active ingredients and through multiple targets and multiple pathways, can stop the growth of cervical cancer cells at a certain phase of the cell cycle and cause apoptosis, which proved CKI's effect in treating cervical cancer.
基于网络药理学和转录组学，研究复方苦参注射液（CKI）在宫颈癌防治中的分子靶点和作用机制。
本研究采用网络药理学方法筛选有效化合物，将网络药理学的结果与 RNA-seq 综合筛选和预测靶基因，分析靶基因的生物学功能和信号通路，并构建 PPI 网络筛选关键基因。通过生物实验、分子对接、RT-PCR 和 Western blot 分析对结果进行进一步验证。
结果表明，CXCL2、抗血管内皮生长因子、己糖激酶 2 等关键基因是 CKI 治疗宫颈癌的治疗靶点。这些靶点主要富集在癌症相关通路、细胞周期、MAPK 信号通路等通路中。体外细胞实验表明，CKI 能有效抑制癌细胞增殖，促进细胞凋亡，诱导细胞周期停滞。RT-PCR 和 Western blot 实验表明关键基因的表达显著降低。化合物对关键基因具有良好的结合活性。
CKI 基于其活性成分，通过多个靶点和多个途径，可在细胞周期的某个阶段阻止宫颈癌细胞的生长并诱导细胞凋亡，从而证实了 CKI 治疗宫颈癌的效果。
Zotero 插件
