College of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, China.
Department of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
J Agric Food Chem. 2023 Sep 20;71(37):13745-13756. doi: 10.1021/acs.jafc.3c01581. Epub 2023 Sep 8.
The apoptosis of intestinal porcine epithelial cells induced by soybean antigen protein allergy is one of the most important mechanisms responsible for enteritis. MicroRNAs (miRNAs) affect the cellular and physiological functions of all multicellular organisms. We hypothesize that microRNA-223 inhibits soybean glycinin- and β-conglycinin-induced apoptosis of intestinal porcine enterocytes (IPEC-J2) by targeting the NLR family pyrin domain containing 3 (). Using the intestinal interepithelial lymphocyte (IEL)/IPEC-J2 co-culture system as an in vitro model, we investigate the role of microRNA-223 in the regulation of soybean glycinin- and β-conglycinin-induced apoptosis. In co-cultured IEL/IPEC-J2 cells incubated with glycinin or β-conglycinin, microRNA-223 decreased NLRP-3, ASC, caspase-1, caspase-3, FAS, BCL-2, and APAF-1 expressions in IPEC-J2 cells; decreased cytokine and cyclooxygenase-2 levels; significantly increased cell activity; and inhibited apoptosis. These data supported a novel antiallergic mechanism to mitigate the sensitization of soybean antigenic protein, which involves the upregulation of microRNA-223-targeting NLRP-3.
大豆抗原蛋白过敏诱导的猪肠上皮细胞凋亡是肠炎的重要发病机制之一。微小 RNA（miRNA）影响所有多细胞生物的细胞和生理功能。我们假设 miR-223 通过靶向 NLR 家族富含pyrin 结构域蛋白 3 () 抑制大豆球蛋白和 β-伴大豆球蛋白诱导的猪肠上皮细胞 (IPEC-J2) 凋亡。我们使用肠上皮内淋巴细胞 (IEL)/IPEC-J2 共培养系统作为体外模型，研究 miR-223 在调节大豆球蛋白和 β-伴大豆球蛋白诱导的凋亡中的作用。在与大豆球蛋白或 β-伴大豆球蛋白孵育的共培养 IEL/IPEC-J2 细胞中，miR-223 降低了 IPEC-J2 细胞中 NLRP-3、ASC、caspase-1、caspase-3、FAS、BCL-2 和 APAF-1 的表达；降低细胞因子和环氧化酶-2 水平；显著增加细胞活性；并抑制细胞凋亡。这些数据支持了一种新的抗过敏机制，可以减轻大豆抗原蛋白的致敏作用，涉及 miR-223 靶向 NLRP-3 的上调。
