The Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen, 361021, China.
The Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen, 361021, China; Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen, 361021, China.
Fish Shellfish Immunol. 2023 Jun;137:108797. doi: 10.1016/j.fsi.2023.108797. Epub 2023 May 5.
β-conglycinin and glycinin, two major heat-stable anti-nutritional factors in soybean meal (SM), have been suggested as the key inducers of intestinal inflammation in aquatic animals. In the present study, a spotted seabass intestinal epithelial cells (IECs) were used to compare the inflammation-inducing effects of β-conglycinin and glycinin. The results showed that IECs co-cultured with 1.0 mg/mL β-conglycinin for 12 h or 1.5 mg/mL glycinin for 24 h significantly decreased the cell viability (P < 0.05), and overstimulated inflammation and apoptosis response by significantly down-regulating anti-inflammatory genes (IL-2, IL-4, IL-10 and TGF-β1) expressions and significantly up-regulated pro-inflammatory genes (IL-1β, IL-8 and TNF-α) and apoptosis genes (caspase 3, caspase 8 and caspase 9) expressions (P < 0.05). Subsequently, a β-conglycinin based inflammation IECs model was established and used for demonstrating whether commensal probiotic B. siamensis LF4 can ameliorate the adverse effects of β-conglycinin. The results showed β-conglycinin-induced cell viability damage was completely repaired by treated with 10 cells/mL heat-killed B. siamensis LF4 for ≥12 h. At the same time, IECs co-cultured with 10 cells/mL heat-killed B. siamensis LF4 for 24 h significantly ameliorated β-conglycinin-induced inflammation and apoptosis by up-regulating anti-inflammatory genes (IL-2, IL-4, IL-10 and TGF-β1) expressions and down-regulated pro-inflammatory genes (IL-1β, IL-8 and TNF-α) and apoptosis genes (caspase 3, caspase 8 and caspase 9) expressions (P < 0.05). In summary, both β-conglycinin and glycinin can lead to inflammation and apoptosis in spotted seabass IECs, and β-conglycinin is more effective; commensal B. siamensis LF4 can efficiently ameliorate β-conglycinin induced inflammation and apoptosis in IECs.
β-伴大豆球蛋白和大豆球蛋白是大豆粕（SM）中两种主要的热稳定抗营养因子，被认为是诱导水产动物肠道炎症的关键因素。本研究以斜带石斑鱼肠上皮细胞（IECs）为模型，比较了β-伴大豆球蛋白和大豆球蛋白的致炎作用。结果表明，与 1.0mg/mLβ-伴大豆球蛋白共培养 12h 或 1.5mg/mL 大豆球蛋白共培养 24h 显著降低了细胞活力（P<0.05），通过显著下调抗炎基因（IL-2、IL-4、IL-10 和 TGF-β1）的表达，过度刺激炎症和凋亡反应，并显著上调促炎基因（IL-1β、IL-8 和 TNF-α）和凋亡基因（caspase 3、caspase 8 和 caspase 9）的表达（P<0.05）。随后，建立了基于β-伴大豆球蛋白的炎症 IECs 模型，并用于验证共生益生菌 B. siamensis LF4 是否能改善β-伴大豆球蛋白的不良作用。结果表明，10 个细胞/mL 热灭活 B. siamensis LF4 处理≥12h 可完全修复β-伴大豆球蛋白诱导的细胞活力损伤。同时，与 10 个细胞/mL 热灭活 B. siamensis LF4 共培养 24h 可通过上调抗炎基因（IL-2、IL-4、IL-10 和 TGF-β1）的表达和下调促炎基因（IL-1β、IL-8 和 TNF-α）和凋亡基因（caspase 3、caspase 8 和 caspase 9）的表达，显著改善β-伴大豆球蛋白诱导的炎症和凋亡（P<0.05）。综上所述，β-伴大豆球蛋白和大豆球蛋白均可导致斜带石斑鱼 IECs 发生炎症和凋亡，且β-伴大豆球蛋白作用更显著；共生益生菌 B. siamensis LF4 可有效改善 IECs 中β-伴大豆球蛋白诱导的炎症和凋亡。
