Zhang Junxiang, Xu Die, Zhang Yan, Luo Zhenzhen, Zhao Yanan, Zheng Xiaolong, Yang Hualin, Zhou Yu
College of Life Science, Yangtze University, 266 Jingmi Road, Jingzhou, Hubei 434025, China.
College of Animal Science and Technology, Yangtze University, 266 Jingmi Road, Jingzhou, Hubei 434025, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jan 5;304:123312. doi: 10.1016/j.saa.2023.123312. Epub 2023 Aug 30.
In this work, a fluorescence immunoassay based on horseradish peroxidase-labeled IgG (HRP-IgG)-modified gold nanoparticle (AuNP@HRP-IgG) probe was established for detection of ochratoxin A (OTA). Through the catalysis of HRP, the dopamine (DA) and 1,5-dihydroxynaphthalene (DHA) can rapidly generate azamonardine fluorescence compound (AFC) with intense yellow fluorescence. Large amounts of AFC can be formed within 4 min, which led to fluorescence enhancement at 545 nm. This new method displayed high sensitivity with a limit of detection (LOD) of 0.18 ng/mL and a linear range of 0.78-200 ng/mL for OTA. Meanwhile, the recoveries of OTA in corn samples were 101.41% - 113.45%. Due to the universality of the probe and the rapidity of signal output, the fluorescence immunoassay allowed rapid and sensitive detection of targets.
在本研究中，建立了一种基于辣根过氧化物酶标记的免疫球蛋白G（HRP-IgG）修饰的金纳米颗粒（AuNP@HRP-IgG）探针的荧光免疫分析法，用于检测赭曲霉毒素A（OTA）。在HRP的催化作用下，多巴胺（DA）和1,5-二羟基萘（DHA）能够快速生成具有强烈黄色荧光的氮杂吖啶荧光化合物（AFC）。4分钟内即可形成大量的AFC，从而导致在545nm处荧光增强。这种新方法具有高灵敏度，OTA的检测限（LOD）为0.18ng/mL，线性范围为0.78-200ng/mL。同时，玉米样品中OTA的回收率为101.41%-113.45%。由于该探针的通用性和信号输出的快速性，该荧光免疫分析法能够快速、灵敏地检测目标物。
