Analytical and Structural Chemistry Department, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500007, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Analytical Division, Institute of Pesticide Formulation Technology (IPFT), Sector-20, Udyog Vihar, Gurugram-122016, Haryana, India.
Food Chem. 2024 Feb 1;433:137342. doi: 10.1016/j.foodchem.2023.137342. Epub 2023 Aug 30.
A new dummy template-based molecularly imprinted dispersive micro solid-phase extraction (MI-d-µSPE) coupled with HPLC-FLD developed for the simultaneous determination of four aflatoxins (B, B, G, G) in various food matrices. The synthesized MIP was used as a dispersive solid-phase extraction (dSPE) sorbent for aflatoxins extraction. The chemometric approach was used to identify the optimum conditions of dSPE. The results showed the amount of MIP sorbent (55 mg), adsorption time (12.5 min), and %ACN (75%) were significant extraction parameters. The method has a detection limit in the range of 0.059-0.208 µg kg and a quantification limit in the range of 0.197-0.694 µg kg for aflatoxins. The intra- and inter-day precision was less than 5%, and recoveries were 79.1-109.4%. The expanded uncertainty of the developed method was found to be 2.9-22.8%. The new MI-d-µSPE with HPLC-FLD method was applied for 37 food matrices.
建立了一种新的基于虚拟模板的分子印迹分散微固相萃取（MI-d-µSPE）与 HPLC-FLD 联用，用于同时测定各种食品基质中的四种黄曲霉毒素（B1、B2、G1、G2）。合成的 MIP 用作黄曲霉毒素萃取的分散固相萃取（dSPE）吸附剂。采用化学计量学方法确定 dSPE 的最佳条件。结果表明，MIP 吸附剂的用量（55mg）、吸附时间（12.5min）和 ACN%（75%）是重要的萃取参数。该方法对黄曲霉毒素的检测限在 0.059-0.208µgkg 范围内，定量限在 0.197-0.694µgkg 范围内。黄曲霉毒素的日内和日间精密度均小于 5%，回收率为 79.1-109.4%。该方法的扩展不确定度为 2.9-22.8%。新的 MI-d-µSPE 与 HPLC-FLD 方法已应用于 37 种食品基质。
