一种新型样品预处理方法，采用纳米纤维填充固相萃取法提取食品中的黄曲霉毒素 B1、B2、G1 和 G2，并与 HPLC 同时测定。

A novel sample pretreatment of nanofiber-packed solid-phase extraction of aflatoxin B1, B2, G1 and G2 in foods and simultaneous determination with HPLC.

机构信息

School of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China.

School of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China; School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

出版信息

Food Chem. 2024 Mar 15;436:137699. doi: 10.1016/j.foodchem.2023.137699. Epub 2023 Oct 20.

DOI:10.1016/j.foodchem.2023.137699

PMID:37866097

Abstract

A novel analytical method based on nanofiber-packed solid-phase extraction (PFSPE) combined with HPLC-FLD has been successfully developed and applied to determine aflatoxin B1, B2, G1, G2 in foods. In order to effectively adsorb analytes from complex food matrices, four types of nanofibers based on skeleton of polystyrene-polyvinyl pyrrolidone were prepared by electrospinning and applied as adsorbent to home-made solid-phase extraction device. The effects of type of nanofibers, pH of sample, type and concentration of salt ion, type of activation solvent, type and volume of eluent, amount of nanofibers, and extraction time on the adsorption/desorption were investigated and optimized. Under optimal conditions, the method showed satisfactory linear relationship, with limits of detection (LODs) of 0.07-0.17 ng g, intra-day and inter-day RSDs for spiked samples of 1.3-8.0 % and 1.9-5.8 %, and absolute recoveries in the range of 60.1-98.4 %. The results presented the great potential to be utilized to determine AFs in foodstuffs.

摘要

一种基于纳米纤维填充固相萃取（PFSPE）结合 HPLC-FLD 的新型分析方法已被成功开发并应用于检测食品中的黄曲霉毒素 B1、B2、G1、G2。为了从复杂的食品基质中有效吸附分析物，通过静电纺丝制备了基于聚苯乙烯-聚乙烯吡咯烷酮骨架的四种纳米纤维，并将其用作自制固相萃取装置的吸附剂。考察并优化了纳米纤维的类型、样品 pH 值、盐离子的类型和浓度、活化溶剂的类型、洗脱剂的类型和体积、纳米纤维的量和萃取时间对吸附/解吸的影响。在最佳条件下，该方法表现出令人满意的线性关系，检出限（LODs）为 0.07-0.17ng/g，加标样品的日内和日间 RSD 分别为 1.3-8.0%和 1.9-5.8%，绝对回收率在 60.1-98.4%范围内。结果表明，该方法具有很大的潜力可用于检测食品中的 AFs。