Lalunio Hannah, Parker Lewan, Hanson Erik D, Gregorevic Paul, Levinger Itamar, Hayes Alan, Goodman Craig A
Department of Medicine, The University of Melbourne, Parkville, Victoria, Australia; Australian Institute for Musculoskeletal Science (AIMSS), Victoria University, St. Albans, Victoria, Australia.
Institute for Physical Activity and Nutrition (IPAN), Deakin University, Geelong, VIC, Australia.
Mol Cell Endocrinol. 2023 Dec 1;578:112050. doi: 10.1016/j.mce.2023.112050. Epub 2023 Sep 7.
Vitamin D, and its receptor (VDR), play roles in muscle development/function, however, VDR detection in muscle has been controversial. Using different sample preparation methods and antibodies, we examined differences in muscle VDR protein abundance between two mouse strains and between mice and humans. The mouse D-6 VDR antibody was not reliable for detecting VDR in mouse muscle, but was suitable for human muscle, while the rabbit D2K6W antibody was valid for mouse and human muscle. VDR protein was generally lower in muscles from C57 B l/6 than FVB/N mice and was higher in human than mouse muscle. Two putative VDR bands were detected in human muscle, possibly representing VDR isoforms/splice variants, with marked inter-individual differences. This study provides new information on detecting VDR in muscle and on inter-mouse strain and inter-human individual differences in VDR expression. These findings may have implications for future pre-clinical and clinical studies and prompt further investigation to confirm possible VDR isoforms in human muscle.
维生素D及其受体（VDR）在肌肉发育/功能中发挥作用，然而，在肌肉中检测VDR一直存在争议。我们使用不同的样品制备方法和抗体，研究了两种小鼠品系之间以及小鼠与人类之间肌肉VDR蛋白丰度的差异。小鼠D - 6 VDR抗体在检测小鼠肌肉中的VDR时不可靠，但适用于人类肌肉，而兔D2K6W抗体对小鼠和人类肌肉均有效。C57 B l/6小鼠肌肉中的VDR蛋白通常低于FVB/N小鼠，且人类肌肉中的VDR蛋白高于小鼠肌肉。在人类肌肉中检测到两条假定的VDR条带，可能代表VDR异构体/剪接变体，个体间差异明显。本研究提供了关于在肌肉中检测VDR以及小鼠品系间和人类个体间VDR表达差异的新信息。这些发现可能对未来的临床前和临床研究具有启示意义，并促使进一步研究以确认人类肌肉中可能存在的VDR异构体。
