Andrade Lucas de Assis Silva, Ortúzar-Ferreira Carlos Nei, Oliveira Mariana de Souza, Cardozo Sergian Vianna, Lima Viviane Moreira de, Berto Bruno Pereira
Programa de Pós-Graduação em Biologia Animal, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, BR-465 km 7, 23897-000 Seropédica, Rio de Janeiro, Brazil.
Programa de Pós-Graduação em Biomedicina Translacional, Universidade do Grande Rio, Rua Professor José de Souza Herdy 1160, 25071-202 Duque de Caxias, Rio de Janeiro, Brazil.
Parasitol Int. 2024 Feb;98:102806. doi: 10.1016/j.parint.2023.102806. Epub 2023 Sep 7.
Chivi vireos Vireo chivi (Vieillot, 1817) are passerine birds widely distributed throughout Brazil, but mainly observed in the Atlantic Forest of the South and Southeast regions of the country. In this context, the current study identifies a new species of Isospora Schneider, 1881 from V. chivi captured in the Marambaia Island, on the coast of the State of Rio de Janeiro, Southeastern Brazil. The oocysts of Isospora juruviarae Andrade & Berto n. sp. are subspheroidal to ovoidal, measuring on average 26 by 24 μm. Micropyle is absent or inconspicuous. Oocyst residuum absent, but polar granules are present. Sporocysts are ellipsoidal with pointed posterior end, measuring on average 17 by × 11 μm. Stieda and Sub-Stieda bodies are present. Sporocyst residuum is present among the vermiform sporozoites, which have refractile bodies and nucleus. This morphology was different from the other Isospora spp. recorded in the same family, superfamily and parvorder as the host. Molecular identification was targeted by the amplification and sequencing of two different loci of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and one locus of the 18S small subunit ribosomal RNA (18S) gene. Phylogenetic analyses were not very efficient in forming monophyletic groups associated with host taxon, zoogeographical region or taxonomic character; however, they confirmed the identification as a new species through comparison with sequences from Isospora spp. of wild passerines. Finally, based on the morphological and molecular analyses of the oocysts recovered from the chivi vireo V. chivi in the current work, I. juruviarae is considered new to science, being the second species recorded in the host family Vireonidae and the first to have a supplementation by molecular identification.
智利绿鹃（Vireo chivi，维约，1817年）是雀形目鸟类，广泛分布于巴西各地，但主要见于该国南部和东南部地区的大西洋森林。在此背景下，本研究从巴西东南部里约热内卢州海岸的马兰巴亚岛捕获的智利绿鹃中鉴定出一种1881年的新等孢球虫属（Isospora Schneider）物种。朱鲁维阿等孢球虫（Isospora juruviarae Andrade & Berto n. sp.）的卵囊呈近球形至卵形，平均大小为26×24μm。微孔不存在或不明显。卵囊残体不存在，但有极粒。孢子囊呈椭圆形，后端尖，平均大小为17×11μm。有斯氏体和亚斯氏体。在具有折光体和细胞核的蠕虫状子孢子之间存在孢子囊残体。这种形态与在同一科、总科和亚目作为宿主记录的其他等孢球虫属物种不同。通过对线粒体细胞色素c氧化酶亚基1（cox1）基因的两个不同位点和18S小亚基核糖体RNA（18S）基因的一个位点进行扩增和测序进行分子鉴定。系统发育分析在形成与宿主分类单元、动物地理区域或分类特征相关的单系类群方面效率不高；然而，通过与野生雀形目等孢球虫属物种的序列比较，它们证实了该物种为新物种。最后，基于本研究中从智利绿鹃中回收的卵囊的形态和分子分析，朱鲁维阿等孢球虫被认为是科学上新发现的物种，是绿鹃科宿主中记录的第二个物种，也是第一个通过分子鉴定得到补充的物种。
