Ortúzar-Ferreira Carlos Nei, Genovez-Oliveira Jhon Lennon, de Souza Oliveira Mariana, de Mello Ericson Ramos, Thode-Filho Sergio, de Oliveira Águida Aparecida, de Lima Viviane Moreira, Ferreira Ildemar, Berto Bruno Pereira
Curso de Graduação em Medicina Veterinária, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brazil.
Programa de Pós-graduação em Biologia Animal, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brazil.
Acta Parasitol. 2020 Dec;65(4):843-851. doi: 10.1007/s11686-020-00237-8. Epub 2020 Jun 3.
Coccidia are obligatory intracellular parasites with at least one intestinal phase in their life cycles, being Isospora Schneider, 1881 the main coccidian genus related to the order Passeriformes. However, there is no record of isosporans from the passerine family Tityridae, which is the family of the greenish schiffornis Schiffornis virescens (Lafresnaye, 1838).
This study aimed to examine the faeces from a greenish schiffornis S. virescens captured in the Itatiaia National Park, State of Rio de Janeiro, Southeastern Brazil, to determine what coccidian parasites were present.
Only one specimen of Schiffornis virescens was captured with mist nets. Coccidian oocysts were recovered from the fecal samples by flotation in Sheather's saturated solution. Morphological observations, line drawings, photomicrographs and measurements were made in optical microscopy and digitally edited. The molecular analysis included the study of the sequence of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene, with phylogenetic reconstructions based on the Neighbor-Joining and Maximum Likelihood analysis.
An Isospora sp. considered as new to science is described and identified from Schiffornis virescens (Lafresnaye, 1838). Isospora oliveirai n. sp. has oocysts that are subspheroidal, 26.0 × 24.8 μm, with rough, bilayered wall, c.2.5 μm thick. Micropyle and oocyst residuum absent, but one to six polar granules are present. Sporocysts lemon-shaped, 18.1 × 10.9 μm. The Stieda body is knob-like to half-moon-shaped and sub-Stieda is rounded. Sporocyst residuum is present, composed of scattered spherules of different sizes. Sporozoites are vermiform, with refractile bodies and nucleus. Molecular analysis at the COI gene exhibited similarity of 97% with Isospora serinuse Yang, Brice, Elliot et Ryan, 2015 from island canaries Serinus canaria (Linnaeus, 1758), and Isospora spp. from great tits Parus major (Linnaeus, 1758) and European robins Erithacus rubecula (Linnaeus, 1758).
Based on the morphological and molecular features, I. oliveirai is considered as new to science and the first coccidian species recorded from Tityridae.
球虫是专性细胞内寄生虫，其生命周期中至少有一个肠道阶段，1881年的等孢属（Isospora Schneider）是与雀形目相关的主要球虫属。然而，关于雀形目蒂泰霸鹟科（Tityridae）却没有等孢属寄生虫的记录，蒂泰霸鹟科是绿翅希夫霸鹟（Schiffornis virescens (Lafresnaye, 1838)）所属的科。
本研究旨在检查在巴西东南部里约热内卢州伊塔伊亚国家公园捕获的一只绿翅希夫霸鹟的粪便，以确定存在哪些球虫寄生虫。
仅用雾网捕获了一只绿翅希夫霸鹟标本。通过在谢弗氏饱和溶液中浮选从粪便样本中回收球虫卵囊。在光学显微镜下进行形态学观察、绘制线条图、拍摄显微照片并进行测量，然后进行数字编辑。分子分析包括对线粒体细胞色素c氧化酶亚基1（COI）基因序列的研究，并基于邻接法和最大似然法进行系统发育重建。
从绿翅希夫霸鹟（Lafresnaye, 1838）中描述并鉴定出一种被认为是科学上新发现的等孢属物种。奥利维拉等孢球虫（Isospora oliveirai n. sp.）的卵囊呈近球形，大小为26.0×24.8μm，壁粗糙，双层，厚约2.5μm。无微孔和卵囊残体，但有1至6个极粒。孢子囊呈柠檬形，大小为18.1×10.9μm。斯氏体呈瘤状至半月形，亚斯氏体呈圆形。有孢子囊残体，由大小不同的散在小球体组成。子孢子呈蠕虫状，有折光体和细胞核。对COI基因的分子分析显示，其与2015年从加那利群岛金丝雀（Serinus canaria (Linnaeus, 1758)）中发现的丝雀等孢球虫（Isospora serinuse Yang, Brice, Elliot et Ryan）、从大山雀（Parus major (Linnaeus, 1758)）和欧歌鸫（Erithacus rubecula (Linnaeus, 1758)）中发现的等孢属物种的相似性为97%。
基于形态学和分子特征，奥利维拉等孢球虫被认为是科学上新发现的物种，也是蒂泰霸鹟科记录的首个球虫物种。
