Ballas S K, Reilly P A, Murphy D L
Biochim Biophys Acta. 1986 Nov 19;884(2):337-43. doi: 10.1016/0304-4165(86)90182-0.
Erythrocytes from twelve individuals with the S-s-U- phenotype and from ten with the S-S-U+ phenotype were analyzed and compared to control cells with S+/s+U+ determinants. No red cell abnormality was detected in S-s-U+ or S-s-U- carriers. Sialic acid content was similar (P greater than 0.05) for S-s-U+ and S-s-U- erythrocytes (74.6 +/- 7.14 and 71.4 +/- 8.53 nmol/10(9) red blood cells, respectively) but significantly less (P less than 0.001) than controls with 89.5 +/- 11.4 nmol/10(9) red blood cells, n = 16. Fluorographs of SDS-polyacrylamide gels showed no glycophorin B in membranes from S-s-U+ and S-s-U- erythrocytes labeled with NaB3H4. Glycophorins were extracted from red cell membranes in chloroform/methanol, labeled with 125I and analyzed by SDS-polyacrylamide gel electrophoresis. Periodic acid Schiff stain and autoradiographs of these gels also showed absence of glycophorin B in both S-s-U+ and S-s-U- cells. These findings suggested that the U antigen is not located on glycophorin B. This hypothesis was tested by determining blood group antigenicity on red cell membranes and on extracted sialoglycoproteins by the hemagglutination inhibition technique. Although U and S/s activities were detected in control red cell membranes, extracted glycoproteins demonstrated S/s activity but no U activity. Together the data indicate that both S-s-U+ and S-s-U- erythrocytes lack glycophorin B and that the U antigen is not located on glycophorin B. This deletion does not seem to affect the structure-function of the red cell.
对12名具有S-s-U-表型的个体和10名具有S-S-U+表型的个体的红细胞进行了分析，并与具有S+/s+U+决定簇的对照细胞进行了比较。在S-s-U+或S-s-U-携带者中未检测到红细胞异常。S-s-U+和S-s-U-红细胞的唾液酸含量相似（P大于0.05）（分别为74.6±7.14和71.4±8.53 nmol/10⁹个红细胞），但显著低于对照组（P小于0.001），对照组为89.5±11.4 nmol/10⁹个红细胞，n = 16。SDS-聚丙烯酰胺凝胶的荧光图谱显示，用NaB³H₄标记的S-s-U+和S-s-U-红细胞膜中没有血型糖蛋白B。血型糖蛋白从红细胞膜中用氯仿/甲醇提取，用¹²⁵I标记并通过SDS-聚丙烯酰胺凝胶电泳分析。这些凝胶的过碘酸希夫染色和放射自显影片也显示，S-s-U+和S-s-U-细胞中均没有血型糖蛋白B。这些发现表明U抗原不在血型糖蛋白B上。通过血凝抑制技术测定红细胞膜和提取的唾液糖蛋白上的血型抗原性，对这一假设进行了检验。虽然在对照红细胞膜中检测到了U和S/s活性，但提取的糖蛋白显示出S/s活性但没有U活性。这些数据共同表明，S-s-U+和S-s-U-红细胞都缺乏血型糖蛋白B，并且U抗原不在血型糖蛋白B上。这种缺失似乎不影响红细胞的结构功能。
