Shinozuka T, Miyata Y, Ohyama K, Takei S, Yamakawa T, Kurihara K, Yanagida J
Department of Legal Medicine, School of Medicine, Keio University, Tokyo, Japan.
Am J Hematol. 1993 Nov;44(3):204-6. doi: 10.1002/ajh.2830440313.
The propositus's erythrocytes with phenotype En(a-), which was found for the first time in a Japanese family, reacted more weakly with anti-N serum than the ordinary phenotype N erythrocytes. The En(a-) erythrocytes lack the major membrane sialoglycoprotein (glycophorin A) as demonstrated by Bio-Gel 1.5m gel filtration from active sialoglycoproteins, which were isolated from En(a-) erythrocyte membranes by the method of lithium diiodosalicylate (LIS)-phenol extraction. It is suggested from observation via enzyme-linked immunosorbent assay (ELISA) that N activity is derived from the glycophorin B molecule on En(a-) erythrocyte membranes.
先证者的红细胞表现型为En(a-)，这是在一个日本家庭中首次发现的，与普通表现型N的红细胞相比，其与抗N血清的反应更弱。通过Bio-Gel 1.5m凝胶过滤从活性唾液酸糖蛋白中分离出的结果表明，En(a-)红细胞缺乏主要的膜唾液酸糖蛋白（血型糖蛋白A），这些活性唾液酸糖蛋白是通过二碘水杨酸锂（LIS）-苯酚萃取法从En(a-)红细胞膜中分离出来的。通过酶联免疫吸附测定（ELISA）观察表明，N活性源自En(a-)红细胞膜上的血型糖蛋白B分子。
