Rabinovich Ia M, Bezborodova O A
Biokhimiia. 1986 Aug;51(8):1322-8.
During incubation of rat liver mitochondria in vitro labeled formate is incorporated into TCA-insoluble substance during mitochondrial translation. Data from hydrolysis with CNBr (after methionine residues) or with 0.5 N HCl (deformylation of amino acid N-formyl derivatives) suggest that about half of the total protein radioactivity is incorporated in formate groups of N-terminal methionine. Labeling of growing polypeptides with formate (but not with phenylalanine or methionine) oscillates with a period of about 13 min. The potential initiation capacity is unchangeable and exceeds that observed experimentally by one order of magnitude. The data obtained are consistent with the previously proposed hypothesis no synchronization of mitochondrial protein synthesis which cannot be induced by the steps preceding the formation of the first peptide bound.
在大鼠肝线粒体的体外孵育过程中，标记的甲酸在翻译过程中被掺入到三羧酸循环（TCA）不溶性物质中。来自用溴化氰（在甲硫氨酸残基之后）或用0.5N盐酸（氨基酸N-甲酰基衍生物的去甲酰化）水解的数据表明，总蛋白质放射性的大约一半被掺入到N-末端甲硫氨酸的甲酸基团中。用甲酸（而不是苯丙氨酸或甲硫氨酸）对生长中的多肽进行标记，其振荡周期约为13分钟。潜在的起始能力是不变的，并且比实验观察到的高一个数量级。所获得的数据与先前提出的关于线粒体蛋白质合成不同步的假设一致，这种不同步不能由第一个肽键形成之前的步骤诱导。
