Smith B D, La Celle P L
Blood. 1986 Nov;68(5):1050-4.
Detachment of individual sickle erythrocytes from cultured endothelial cell monolayers has been evaluated by a fluid-shearing technique in an effort to quantitate adherence at shear forces that would be anticipated in the in vivo circulation. Nonirreversibly sickled cells (non-ISC) were more adherent at normal oxygen tensions than control cells. More than 1% non-ISC remained attached to the monolayer at forces greater than physiologic shear stresses in capillary and venous circulations, and many of the most avidly attached cells, once separated, immediately reattached to adjacent endothelial cells. These data suggest that hemoglobin S-containing erythrocytes may have a higher frequency of adherence in vivo in regions of low shear stress where prolonged erythrocyte-endothelial cell contact could occur. Some of these cells detached by shear force would subsequently reattach in in vivo conditions. Plasma-enhanced attachment frequency and plasma from blood in a case of sickle crisis caused further increase. These observations further support the concept that sickle erythrocyte-endothelial cell interaction may be a significant factor in initiation of vascular occlusive events in sickle cell disease.
通过流体剪切技术评估了从培养的内皮细胞单层中分离出的单个镰状红细胞，旨在定量在体内循环中预期的剪切力下的黏附情况。在正常氧张力下，非不可逆镰状细胞（non-ISC）比对照细胞更具黏附性。在大于毛细血管和静脉循环中生理剪切应力的力作用下，超过1%的非ISC仍附着于单层，并且许多黏附最紧密的细胞一旦分离，会立即重新附着于相邻的内皮细胞。这些数据表明，含血红蛋白S的红细胞在体内低剪切应力区域可能具有更高的黏附频率，在这些区域可能会发生红细胞与内皮细胞的长时间接触。一些通过剪切力分离的细胞随后会在体内条件下重新附着。血浆增强了黏附频率，镰状细胞危象患者血液中的血浆进一步增加了黏附频率。这些观察结果进一步支持了这样一种概念，即镰状红细胞与内皮细胞的相互作用可能是镰状细胞病血管闭塞事件发生的一个重要因素。
