GECORP, Av. Juan Manuel de Rosas 899, Monte, Buenos Aires 7220, Argentina.
Department of Clinical Biochemistry, Aalborg University Hospital, Aalborg Hobrovej 18-22, 9000 Aalborg, Denmark.
Int J Mol Sci. 2023 Aug 28;24(17):13326. doi: 10.3390/ijms241713326.
Non-alcoholic fatty liver disease (NAFLD) is a liver disorder that has become a global health concern due to its increasing prevalence. There is a need for reliable biomarkers to aid in the diagnosis and prognosis of NAFLD. Extracellular vesicles (EVs) are promising candidates in biomarker discovery, as they carry proteins that reflect the pathophysiological state of the liver. In this review, we developed a list of EV proteins that could be used as diagnostic biomarkers for NAFLD. We employed a multi-step strategy that involved reviewing and comparing various sources of information. Firstly, we reviewed papers that have studied EVs proteins as biomarkers in NAFLD and papers that have studied circulating proteins as biomarkers in NAFLD. To further identify potential candidates, we utilized the EV database Vesiclepedia.org to qualify each protein. Finally, we consulted the Human Protein Atlas to search for candidates' localization, focusing on membrane proteins. By integrating these sources of information, we developed a comprehensive list of potential EVs membrane protein biomarkers that could aid in diagnosing and monitoring NAFLD. In conclusion, our multi-step strategy for identifying EV-based protein biomarkers for NAFLD provides a comprehensive approach that can also be applied to other diseases. The protein candidates identified through this approach could have significant implications for the development of non-invasive diagnostic tests for NAFLD and improve the management and treatment of this prevalent liver disorder.
非酒精性脂肪性肝病（NAFLD）是一种肝脏疾病，由于其发病率不断上升，已成为全球关注的健康问题。因此，需要可靠的生物标志物来辅助 NAFLD 的诊断和预后。细胞外囊泡（EVs）作为生物标志物发现的候选者具有很大的潜力，因为它们携带的蛋白质反映了肝脏的病理生理状态。在本综述中，我们列出了一些可能作为 NAFLD 诊断生物标志物的 EV 蛋白。我们采用了一种多步骤策略，涉及审查和比较各种来源的信息。首先，我们回顾了研究 EV 蛋白作为 NAFLD 生物标志物的论文，以及研究循环蛋白作为 NAFLD 生物标志物的论文。为了进一步确定潜在的候选者，我们利用 EV 数据库 Vesiclepedia.org 来对每个蛋白进行定性。最后，我们查阅了人类蛋白质图谱，以寻找候选蛋白的定位，重点是膜蛋白。通过整合这些信息来源，我们开发了一个潜在的 EV 膜蛋白生物标志物的综合列表，这些标志物可能有助于诊断和监测 NAFLD。总之，我们用于鉴定基于 EV 的 NAFLD 蛋白生物标志物的多步骤策略提供了一种全面的方法，也可应用于其他疾病。通过这种方法鉴定的候选蛋白可能对开发非侵入性诊断测试用于 NAFLD 具有重要意义，并能改善这种常见肝脏疾病的管理和治疗。
