Technical Research Center of Dry Crop Variety Breeding in Fujian Province, Crop Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China.
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
Int J Mol Sci. 2023 Aug 30;24(17):13496. doi: 10.3390/ijms241713496.
The pollution of heavy metals is extremely serious in China, including zinc (Zn), copper (Cu), lead (Pb), and cadmium (Cd). Heavy-metal-transporting ATPase (HMA) belongs to a subfamily of the P-ATPase family, which absorbs and transports Zn, Cu, Pb, and Cd in plants. Here, we describe a -encoding HMA family protein that positively regulates Cd and Zn tolerance. The real-time fluorescence quantification (RT-PCR) results revealed that had a high expression in B73, and the expression of was sensitive to Cd in yeast cells, which was related to Cd accumulation in yeast. Additionally, the homologous mutants of showed Cd sensitivity compared with WT. The overexpressing plants showed higher tolerance under Cd and Zn stresses than the wild type. The overexpression of led to higher Cd and Zn accumulation in tissues based on the subcellular distribution analysis. We propose that improves maize tolerance to Cd and Zn stresses by absorbing and transporting Cd and Zn ions. This study elucidates the gene function of the response to Cd and Zn stress and provides a reference for improving the characteristics of heavy metals enrichment in existing maize varieties and the plant remediation technology of heavy-metal-contaminated soil.
中国重金属污染十分严重，包括锌（Zn）、铜（Cu）、铅（Pb）和镉（Cd）。重金属转运 ATP 酶（HMA）属于 P-ATP 酶家族的一个亚家族，它在植物中吸收和转运 Zn、Cu、Pb 和 Cd。在这里，我们描述了一个编码 HMA 家族蛋白的基因，该基因正向调控 Cd 和 Zn 耐受性。实时荧光定量（RT-PCR）结果显示， 在 B73 中高表达， 在酵母细胞中对 Cd 敏感，与酵母中 Cd 积累有关。此外， 与 WT 相比，同源突变体的 Cd 敏感性更高。过表达 的植株在 Cd 和 Zn 胁迫下表现出更高的耐受性。基于亚细胞分布分析，过表达 导致组织中 Cd 和 Zn 积累增加。我们提出， 通过吸收和转运 Cd 和 Zn 离子， 提高了玉米对 Cd 和 Zn 胁迫的耐受性。本研究阐明了 对 Cd 和 Zn 胁迫的基因功能，为提高现有玉米品种对重金属的富集特性和重金属污染土壤的植物修复技术提供了参考。
