Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang 571339, China.
Department of Plant and Agroecosytem Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
Int J Mol Sci. 2023 Sep 4;24(17):13658. doi: 10.3390/ijms241713658.
The (SQUAMOSA-promoter binding protein-like) gene family is one of the largest plant transcription factors and is known to be involved in the regulation of plant growth, development, and stress responses. The genome-wide analysis of gene members in a diverse range of crops has been elucidated. However, none of the genome-wide studies on the gene family have been carried out for oil palm, an important oil-yielding plant. In this research, a total of 24 genes were identified via a genome-wide approach. Phylogenetic analysis revealed that most of the s are closely related to the Arabidopsis and rice gene members. genes were mapped onto the only nine chromosomes of the oil palm genome. Motif analysis revealed conservation of the SBP domain and the occurrence of 1-10 motifs in gene members. Gene duplication analysis demonstrated the tandem duplication of members in the oil palm genome. Heatmap analysis indicated the significant expression of genes in shoot and flower organs of oil palm plants. Among the identified genes, a total 14 s were shown to be targets of miR156. Real-time PCR analysis of 14 genes showed that most of the genes were more highly expressed in female and male inflorescences of oil palm plants than in vegetative tissues. Altogether, the present study revealed the significant role of genes in inflorescence development.
（SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE）基因家族是最大的植物转录因子之一，已知参与植物生长、发育和应激反应的调节。已经阐明了在各种作物中对基因成员进行的全基因组分析。然而，对于油棕这一重要的产油植物，尚未进行全基因组范围内对基因家族的研究。在这项研究中，通过全基因组方法共鉴定出 24 个基因。系统发育分析表明，大多数 s 与拟南芥和水稻的基因成员密切相关。基因被映射到油棕基因组的仅 9 条染色体上。基序分析表明 SBP 结构域的保守性和基因成员中 1-10 个基序的发生。基因复制分析表明油棕基因组中基因成员的串联重复。热图分析表明基因在油棕植物的芽和花器官中的显著表达。在所鉴定的基因中，共有 14 个基因被证明是 miR156 的靶基因。对 14 个基因的实时 PCR 分析表明，大多数基因在油棕植物的雌性和雄性花序中的表达高于营养组织。总的来说，本研究揭示了基因在花序发育中的重要作用。
