Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing 102206, China.
College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206, China.
Int J Mol Sci. 2019 Mar 29;20(7):1577. doi: 10.3390/ijms20071577.
Chestnut () is a deciduous tree species with major economic and ecological value that is widely used in the study of floral development in woody plants due its monoecious and out-of-proportion characteristics. Squamosa promoter-binding protein-like () is a plant-specific transcription factor that plays an important role in floral development. In this study, a total of 18 genes were identified in the chestnut genome, of which 10 genes have complementary regions of . An analysis of the phylogenetic tree of the squamosa promoter-binding protein (SBP) domains of the genes of , , and divided these genes into eight groups. The evolutionary relationship between poplar and chestnut in the same group was similar. A structural analysis of the protein-coding regions (CDSs) showed that the domains have the main function of SBP domains and that other domains also play an important role in determining gene function. The expression patterns of and in different floral organs of chestnut were analyzed by real-time quantitative PCR. Some with similar structural patterns showed similar expression patterns, indicating that the gene structures determine the synergy of the gene functions. The application of gibberellin (GA) and its inhibitor (Paclobutrazol, PP) to chestnut trees revealed that these exert a significant effect on the number and length of the male and female chestnut flowers. GA treatment significantly increased expression and thus significantly decreased the expression of its target gene, //, during floral bud development. This finding indicates that GA might indirectly affect the expression of some of the target genes through miR156. In addition, RNA ligase-mediated rapid amplification of the 5' cDNA ends (RLM-RACE) experiments revealed that cleaves and at the 10th and 12th bases of the complementary region. These results laid an important foundation for further study of the biological function of in the floral development of .
栗树是一种具有重要经济和生态价值的落叶树种，因其雌雄同株和不成比例的特点，广泛应用于木本植物花发育的研究。Squamosa 启动子结合蛋白样（SBP）是一种植物特异性转录因子，在花发育中发挥重要作用。本研究在栗树基因组中鉴定出 18 个基因，其中 10 个基因具有 互补区。对 、 、 的 SBP 结构域基因的系统发育树分析将这些基因分为 8 组。同组杨树和栗树的进化关系相似。对蛋白编码区（CDS）的结构分析表明，该结构域具有 SBP 结构域的主要功能，其他结构域也在决定基因功能方面发挥重要作用。通过实时定量 PCR 分析 、 在栗树不同花器官中的表达模式。一些具有相似结构模式的 表现出相似的表达模式，表明基因结构决定了基因功能的协同作用。用赤霉素（GA）及其抑制剂（多效唑，PP）处理栗树表明，这些处理对雄花和雌花的数量和长度有显著影响。GA 处理显著增加了 的表达，从而显著降低了其靶基因 // 在花芽发育过程中的表达。这一发现表明，GA 可能通过 miR156 间接影响一些 靶基因的表达。此外，RNA 连接酶介导的 5' cDNA 末端快速扩增（RLM-RACE）实验表明， 在互补区的第 10 位和第 12 位切割 和 。这些结果为进一步研究 在栗树花发育中的生物学功能奠定了重要基础。
