Mauceri Rodolfo, Coppini Martina, Vacca Davide, Bertolazzi Giorgio, Cancila Valeria, Tripodo Claudio, Campisi Giuseppina
Department of Surgical, Oncological and Oral Sciences, University of Palermo, 90127 Palermo, Italy.
Unit of Oral Medicine and Dentistry for Frail Patients, Department of Rehabilitation, Fragility and Continuity of Care, University Hospital Palermo, 90127 Palermo, Italy.
Cancers (Basel). 2023 Aug 22;15(17):4211. doi: 10.3390/cancers15174211.
Advancements in DNA sequencing technology have facilitated the assessment of the connection between the oral microbiome and various diseases. The aim of the present study was to investigate the salivary microbiota composition employing for the first time in the literature the Oxford Nanopore Technology in patients affected by oral squamous cell carcinoma (OSCC).
Unstimulated saliva samples of 31 patients were collected (24 OSCC patients and 7 controls). DNA was extracted using the QIAamp DNA Blood Kit and metagenomic long sequencing reads were performed using the MinION device.
In the OSCC group, 13 were males and 11 were females, with a mean age of 65.5 ± 13.9 years; in the control group, 5 were males and 2 were females, with a mean age of 51.4 ± 19.2 years. The border of the tongue was the most affected OSCC site. The microorganisms predominantly detected in OSCC patients were , , , , , and .
This study confirmed the predominance of periodontopathic bacteria in the salivary microbiome in the OSCC group. If a direct correlation between oral dysbiosis and OSCC onset was proven, it could lead to new prevention strategies and early diagnostic tools.
DNA测序技术的进步促进了对口腔微生物群与各种疾病之间联系的评估。本研究的目的是首次在文献中运用牛津纳米孔技术，调查口腔鳞状细胞癌（OSCC）患者的唾液微生物群组成。
收集31名患者（24名OSCC患者和7名对照）的非刺激性唾液样本。使用QIAamp DNA血液试剂盒提取DNA，并使用MinION设备进行宏基因组长测序读数。
在OSCC组中，男性13名，女性11名，平均年龄为65.5±13.9岁；在对照组中，男性5名，女性2名，平均年龄为51.4±19.2岁。舌边缘是受OSCC影响最严重的部位。在OSCC患者中主要检测到的微生物是 、 、 、 、 、 和 。
本研究证实了OSCC组唾液微生物群中牙周病原菌占主导地位。如果口腔生态失调与OSCC发病之间的直接相关性得到证实，可能会带来新的预防策略和早期诊断工具。
