维生素 A 通过 RARα 促进小鼠骨骼肌损伤修复。
Vitamin A Promotes the Repair of Mice Skeletal Muscle Injury through RARα.
机构信息
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China.
Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin 150030, China.
出版信息
Nutrients. 2023 Aug 22;15(17):3674. doi: 10.3390/nu15173674.
Vitamin A (VitA) is an important fat-soluble vitamin which plays an important role in cell growth and individual development. However, the effect of VitA on the repair process of muscle injury and its molecular mechanism are still unclear. In this study, VitA and RA were first added to the culture medium of differentiated cells. We then detected cell differentiation marker proteins and myotube fusion. Moreover, the effects of VitA on RARα expression and nuclear translocation were further examined. The results showed that VitA significantly promoted the differentiation of C2C12, and the expression of RARα was significantly increased. Furthermore, VitA was injected into skeletal muscle injury in mice. HE staining and Western Blot results showed that VitA could significantly accelerate the repair of skeletal muscle injury and VitA increase the expression of RARα in mice. This study provides a theoretical basis for elucidating the regulation mechanism of VitA-mediated muscle development and the development of therapeutic drugs for muscle diseases in animals.
维生素 A（VitA）是一种重要的脂溶性维生素，在细胞生长和个体发育中发挥着重要作用。然而，VitA 对肌肉损伤修复过程的影响及其分子机制尚不清楚。在本研究中，首先在分化细胞的培养基中添加 VitA 和 RA，然后检测细胞分化标志物蛋白和肌管融合。此外，还进一步研究了 VitA 对 RARα表达和核转位的影响。结果表明，VitA 能显著促进 C2C12 的分化，RARα 的表达明显增加。进一步将 VitA 注射到小鼠的骨骼肌损伤部位，HE 染色和 Western Blot 结果表明，VitA 能显著加速骨骼肌损伤的修复，且 VitA 能增加小鼠体内 RARα 的表达。本研究为阐明 VitA 介导的肌肉发育调控机制以及动物肌肉疾病治疗药物的开发提供了理论依据。
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