Laboratório de Transformação de Plantas e Análise em Microscopia (LTPAM), Departamento de Biologia Celular e Genética, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-970, RN, Brazil.
Programa de Pós-graduação em Bioquímica e Biologia Molecular, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-970, RN, Brazil.
Nutrients. 2023 Sep 4;15(17):3855. doi: 10.3390/nu15173855.
Medicinal plants, such as , are rich in antioxidant biomolecules, which are used in the treatment and prevention of many diseases. The antioxidant potential of extracts obtained from leaves and fruit peels was investigated using biochemical and 3T3 cell line assays as well as in vivo assays using an organism model . Four extracts were tested: hydroethanolic extracts from leaves (HF) and from fruit peels (HC), and infusion extracts from leaves (IF) and from fruit peels (IC). The biochemical assays demonstrated an antioxidant capacity verified by TAC, reducing power, DPPH, and copper chelating assays. None of the extracts exhibited cytotoxicity against 3T3 cells, instead offering a protection against CuSO-induced oxidative stress. The antioxidant activity observed in the extracts, including their role as free radical scavengers, copper chelators, and stress protectors, was further confirmed by assays. The CLAE-DAD analysis detected phenolic compounds, including gallic acid, rutin, and quercitrin, as the main constituents of the samples. This study highlights that leaf and fruit peels extracts of could be effective protectors against ROS and copper-induced stress in cellular and invertebrate models, and they should be considered as coadjutants in the treatment and prevention of diseases related to oxidative stress and for the development of natural nutraceutical products.
药用植物,如 ,富含抗氧化生物分子,用于治疗和预防许多疾病。使用生化和 3T3 细胞系测定以及使用生物体模型进行的体内测定研究了来自叶和果皮的 提取物的抗氧化潜力 。测试了四种提取物:叶(HF)和果皮(HC)的水醇提取物,以及叶(IF)和果皮(IC)的浸膏提取物。生化测定表明,TAC、还原能力、DPPH 和铜螯合测定证实了抗氧化能力。四种提取物均未对 3T3 细胞表现出细胞毒性,反而对 CuSO 诱导的氧化应激具有保护作用。提取物中的抗氧化活性,包括其作为自由基清除剂、铜螯合剂和应激保护剂的作用,通过 测定进一步得到证实。CLAE-DAD 分析检测到了酚类化合物,包括没食子酸、芦丁和槲皮苷,它们是样品的主要成分。本研究强调, 和果皮的叶提取物可有效保护细胞和无脊椎动物模型免受 ROS 和铜诱导的应激,可作为治疗和预防与氧化应激相关疾病以及开发天然营养保健品的辅助剂。