Laboratório de Transformação de Plantas e Análise Em Microscopia (LTPAM), Departamento de Biologia Celular e Genética, Universidade Federal Do Rio Grande Do Norte (UFRN), Natal, RN, Brazil; Programa de Pós-graduação Em Bioquímica e Biologia Molecular, Centro de Biociências, UFRN, Natal, RN, Brazil.
Programa de Pós-graduação Em Bioquímica e Biologia Molecular, Centro de Biociências, UFRN, Natal, RN, Brazil; Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Universidade Federal Do Rio Grande Do Norte (UFRN), Natal, RN, Brazil.
J Ethnopharmacol. 2025 Jan 30;337(Pt 3):118932. doi: 10.1016/j.jep.2024.118932. Epub 2024 Oct 10.
Talisia esculenta is a fruit tree commonly found in various regions of Brazil. Its fruit is consumed by the local population, and the leaves are used in infusions within traditional Brazilian medicine. These infusions are employed to alleviate pathological conditions such as rheumatic diseases and hypertension, both of which are strongly linked to oxidative stress and chronic inflammation. The investigation of plant extracts represents a promising field of research, as bioactive compounds abundant in plants exhibit pharmacological effects against a variety of pathological conditions.
To investigate the antioxidant, anti-inflammatory activities, and toxicity of the infusion and hydroethanolic extracts of T. esculenta leaves (IF and HF) and fruit peels (IC and HC).
Initially, the cytotoxicity and the effects of the extracts on oxidative stress in RAW264.7 macrophages were assessed through exposure to H₂O₂, as well as their impact on NO production in RAW264.7 macrophages exposed to LPS. Additionally, the toxicity and ROS production in zebrafish larvae were evaluated using two oxidative stress inducers: H₂O₂ and CuSO₄ combined with ascorbate.
The MTT assay indicated that the extracts exhibited low cytotoxicity, with HF and IF demonstrating protective effects against H₂O₂ exposure. HC reduced NO production in macrophages by 30%. The zebrafish analysis showed that all four T. esculenta extracts (100 μg/mL) were non-toxic, as they did not affect the survival, heart rate, or body size of the animals. Furthermore, all extracts were capable of reducing ROS levels in zebrafish larvae exposed to the H₂O₂ stressor. Notably, ROS reduction by HF, IF, and HC extracts exceeded 50% compared to the positive control (H₂O₂ alone). T. esculenta extracts also demonstrated a significant ability to reduce ROS levels in zebrafish larvae exposed to CuSO₄, with a 70% reduction observed for leaf extracts and over 30% for fruit peel extracts.
This study demonstrated that T. esculenta extracts exhibit significant activity against oxidative damage and contain components with anti-inflammatory properties. Among the extracts, those obtained from leaves were the most effective in providing oxidative protection, supporting the traditional use of leaf infusions.
Talisia esculenta 是一种在巴西各地广泛存在的果树。其果实被当地居民食用，而叶子则被用于巴西传统医学中的汤剂。这些汤剂被用于缓解风湿性疾病和高血压等病理状况，这些疾病都与氧化应激和慢性炎症密切相关。植物提取物的研究具有广阔的前景，因为植物中富含的生物活性化合物对多种病理状况具有药理学作用。
研究 Talisia esculenta 叶的汤剂和水醇提取物（IF 和 HF）以及果皮的汤剂和水醇提取物（IC 和 HC）的抗氧化、抗炎活性和毒性。
首先，通过暴露于 H₂O₂评估提取物对 RAW264.7 巨噬细胞的细胞毒性和对氧化应激的影响，以及它们对 LPS 暴露的 RAW264.7 巨噬细胞中 NO 产生的影响。此外，使用两种氧化应激诱导剂（H₂O₂和 CuSO₄与抗坏血酸）评估了在斑马鱼幼虫中的毒性和 ROS 产生。
MTT 测定表明，提取物的细胞毒性较低，HF 和 IF 对 H₂O₂暴露具有保护作用。HC 降低了巨噬细胞中 30%的 NO 产生。斑马鱼分析表明，四种 Talisia esculenta 提取物（100μg/mL）均无毒性，因为它们不会影响动物的存活率、心率或体型。此外，所有提取物都能够降低暴露于 H₂O₂应激源的斑马鱼幼虫中的 ROS 水平。值得注意的是，HF、IF 和 HC 提取物降低 ROS 水平的效果超过 50%，优于阳性对照（单独的 H₂O₂）。Talisia esculenta 提取物还表现出显著降低暴露于 CuSO₄的斑马鱼幼虫中 ROS 水平的能力，叶片提取物降低了 70%，果皮提取物降低了 30%以上。
本研究表明，Talisia esculenta 提取物对氧化损伤具有显著的活性，并且含有具有抗炎特性的成分。在提取物中，来自叶子的提取物在提供氧化保护方面最为有效，支持了叶子汤剂的传统用途。
