Anderson W A, Flumerfelt B A
Brain Res. 1986 Sep 24;383(1-2):228-44. doi: 10.1016/0006-8993(86)90022-3.
The climbing fiber input to the cerebellar cortex was destroyed using both electrolytic and chemical (3-acetylpyridine) lesions. The long-term effects of climbing fiber deafferentation on the ansiform lobule of weanling and adult rats were examined at both the light and electron microscopic levels. Image analysis of Golgi-impregnated Purkinje cells indicated a significantly lower number of smooth branches and spiny branchlets following climbing fiber deafferentation of both adult and weanling rats. The results suggest that the lower number of smooth branches and spiny branchlets following climbing fiber deafferentation of the weanling rat is the result of a loss of postnatal growth rather than transneuronal degeneration. Ultrastructural evidence is provided in confirmation of these quantitative findings. Formation of ectopic dendritic spines was found following climbing fiber deafferentation of the weanling rat, but not the adult. It is shown that ectopic spines and the denervated dendritic thorns of these animals were synaptically innervated by the parallel fiber system and basket axons. The formation of ectopic spines on climbing fiber deafferentated Purkinje cells may represent a form of dendritic plasticity. Ultrastructurally, the dendritic arborizations of weanling deafferentated Purkinje cells showed no signs of transneuronal degeneration. However, the primary response to climbing fiber deafferentation in the adult rat was marked transneuronal degeneration of the Purkinje cell dendrites. It is suggested that the inability of the adult Purkinje cell to form ectopic spines and to replace the excitatory postsynaptic potential of the climbing fiber varicosity is directly related to the Purkinje cell's subsequent transneuronal degeneration.
采用电解损伤和化学（3-乙酰吡啶）损伤相结合的方法破坏了输入到小脑皮质的攀缘纤维。在光镜和电镜水平上研究了攀缘纤维脱失对断奶大鼠和成年大鼠袢小叶的长期影响。对高尔基染色的浦肯野细胞进行图像分析表明，成年和断奶大鼠在攀缘纤维脱失后，平滑分支和棘状小分支的数量均显著减少。结果表明，断奶大鼠攀缘纤维脱失后平滑分支和棘状小分支数量减少是出生后生长丧失的结果，而非跨神经元变性。提供了超微结构证据来证实这些定量研究结果。在断奶大鼠而非成年大鼠的攀缘纤维脱失后发现了异位树突棘的形成。结果表明，这些动物的异位棘和去神经支配的树突棘由平行纤维系统和篮状轴突进行突触支配。在攀缘纤维脱失的浦肯野细胞上形成异位棘可能代表了一种树突可塑性形式。在超微结构上，断奶后脱失的浦肯野细胞的树突分支没有显示出跨神经元变性的迹象。然而，成年大鼠对攀缘纤维脱失的主要反应是浦肯野细胞树突的明显跨神经元变性。有人提出，成年浦肯野细胞无法形成异位棘并替代攀缘纤维曲张体的兴奋性突触后电位，这与浦肯野细胞随后的跨神经元变性直接相关。
