Anderson W A, Flumerfelt B A
Brain Res. 1984 Feb;314(2):285-91. doi: 10.1016/0165-3806(84)90050-6.
Climbing fiber deafferentation of the weanling rat was performed initially by means of intraperitoneal administration of 3-acetylpyridine (3-AP, 65 mg/kg). This dosage of 3-AP, which has been used routinely to achieve olivary ablations in the adult rat, is inappropriate for the total destruction of the inferior olivary complex in weanling rats (21-23 days). Examination of the inferior olivary complex at both the light and electron microscopic levels has revealed a consistent sparing of neurons within the nucleus beta, the genu of the principal nucleus and the caudal portion of the medial accessory olive following long-term (120 days) 3-AP treatment. Complete olivary ablations in weanling rats were obtained only after the injection of a higher dose of 3-AP (90 mg/kg). It would appear that immature inferior olivary neurons are less sensitive to the toxic effect of 3-AP but become increasingly susceptible to 3-AP with maturation. If the appropriate dosage of 3-AP is employed, however, it can be used reliably to produce a chemical ablation of the total climbing fiber input to the cerebellar cortex in weanling rats.
最初通过腹腔注射3-乙酰吡啶（3-AP，65毫克/千克）对断乳大鼠进行攀缘纤维去传入操作。这个剂量的3-AP一直被常规用于成年大鼠的橄榄核损毁，但对于断乳大鼠（21 - 23日龄）的下橄榄复合体的完全破坏来说并不合适。在光镜和电镜水平对下橄榄复合体进行检查发现，经过长期（120天）的3-AP处理后，β核、主核膝部和内侧副橄榄尾部的神经元始终有留存。只有在注射更高剂量的3-AP（90毫克/千克）后，才实现了断乳大鼠橄榄核的完全损毁。似乎未成熟的下橄榄神经元对3-AP的毒性作用不太敏感，但随着成熟会越来越容易受到3-AP的影响。然而，如果采用合适剂量的3-AP，它可以可靠地用于对断乳大鼠小脑皮质的全部攀缘纤维输入进行化学损毁。
