Department of Biochemistry, Faculty of Agriculture, Benha University, Moshtohor 13736, Egypt.
Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig 44519, Egypt.
Molecules. 2023 Sep 1;28(17):6389. doi: 10.3390/molecules28176389.
This study aimed to investigate the phenolic and antioxidant properties of Egyptian leaves extract (SOE) while comparing the antihyperglycemic efficacy of SOE with that of conventional medicines (glibenclamide) in vivo as a substitution for insulin-deficient patients. Total phenolic (TPC) and flavonoid contents (TFC) in SOE contributed around 127.66 ± 0.56 mg GAE/gm as gallic acid equivalent (GAE) and 74.80 ± 0.55 mg QE/gm as quercetin equivalent (QE). SOE also showed significant DPPH scavenging activity at 43.46%. The presence of five phenolic and six flavonoid compounds in SOE was discovered by HPLC analysis. For the in vivo assay, 42 rats were distributed into six groups (7 Wister albino rats each). The standard control group was fed a basal diet. While the 35 rats were induced with a single dose of 100 mg kg body weight (b.w.) alloxan, then treated orally with glibenclamide (GLI) at 10 mg kg, 100, 200, and 300 mg kg SOE (positive control group) for 56 days of routine gastric oral gavages and compared to the effects of GLI, the treatment of SOE 200 and 300 mg kg in diabetic rats for two months dramatically decreased blood glucose, total lipid, total cholesterol, and low-density lipoprotein cholesterol (LDLC) while boosting high-density lipoprotein cholesterol (HDLC) levels and improving liver and kidney functions. The histological assay revealed that the SOE 300 mg kg treatment significantly improved the pancreas tissues, implying the potential application of Egyptian SOE as a diabetes treatment.
本研究旨在探讨埃及叶提取物（SOE）的酚类和抗氧化特性，并将 SOE 的抗高血糖功效与常规药物（格列本脲）进行体内比较，作为胰岛素缺乏患者的替代药物。SOE 中的总酚（TPC）和类黄酮含量（TFC）分别约为 127.66±0.56mg GAE/gm 以没食子酸当量（GAE）和 74.80±0.55mg QE/gm 以槲皮素当量（QE）表示。SOE 对 DPPH 也具有显著的清除活性，达到 43.46%。通过 HPLC 分析发现 SOE 中存在五种酚类和六种类黄酮化合物。在体内试验中，将 42 只大鼠分为六组（每组 7 只 Wister 白化大鼠）。标准对照组给予基础饮食。35 只大鼠用 100mg kg 体重（bw）一次性注射链脲佐菌素诱导，然后用格列本脲（GLI）10mg kg、100、200 和 300mg kg SOE（阳性对照组）口服治疗，常规胃口服灌胃 56 天，并与 GLI 治疗效果进行比较，SOE 200 和 300mg kg 治疗两个月可显著降低糖尿病大鼠的血糖、总脂质、总胆固醇和低密度脂蛋白胆固醇（LDLC），同时提高高密度脂蛋白胆固醇（HDLC）水平，并改善肝肾功能。组织学分析表明，SOE 300mg kg 治疗可显著改善胰腺组织，这意味着埃及 SOE 具有作为糖尿病治疗的潜在应用价值。
