Department of Pharmacy, Al-Yarmok University College, Diyala, Iraq.
Department of Pharmacy, Bilad Alrafidain University College, Diyala, Iraq.
Asian Pac J Cancer Prev. 2024 Oct 1;25(10):3643-3649. doi: 10.31557/APJCP.2024.25.10.3643.
The present study aimed to evaluate the efficacy of the Cnicus benedictus leaf methanolic extract (CBHE) in reducing the growth of cervical cancer cells (Hela cancer cell line).
The extraction was achieved using the Soxhlet apparatus. The study utilized a human cervical cancer cell line for antiproliferative evaluation and a human fibroblast cell line for toxicity assessment on normal cells. The incubation periods were 24 and 72 hours, and the concentration of the extract varied between 0.1 and 1,000 µg/ml.
The study exhibits that the methanolic extract of Cnicus Benedictus leaves can lessen the growth of human cervical cancer cells. The growth inhibition of the extract was dependent on the concentration and time, with the highest inhibition rate seen at 1,000 µg/ml after 72 hours of incubation. The study also revealed that the extract had minimal impact on the growth of normal cells.
The study shows that Cnicus Benedictus leaves methanolic extract has ability to inhibit the growth of human cervical cancer cells in vitro. The extract cytotoxic behavior was (cell cycle and cell non-cycle) specific. The research also found that the extract selectively kills cancer cells rather than normal cells, indicating its safety in their effectivity.
本研究旨在评估苍术叶甲醇提取物（CBHE）在抑制宫颈癌（人宫颈癌细胞系）生长方面的功效。
采用索氏提取器进行提取。研究采用人宫颈癌细胞系进行抗增殖评价，用人成纤维细胞系进行正常细胞毒性评估。孵育期为 24 小时和 72 小时，提取物的浓度在 0.1 至 1000µg/ml 之间变化。
本研究表明苍术叶甲醇提取物可降低人宫颈癌细胞的生长。提取物的生长抑制作用取决于浓度和时间，在 72 小时孵育后 1000µg/ml 时抑制率最高。研究还表明，提取物对正常细胞的生长影响极小。
本研究表明苍术叶甲醇提取物具有抑制人宫颈癌细胞体外生长的能力。提取物的细胞毒性行为是（细胞周期和非细胞周期）特异性的。研究还发现，提取物选择性地杀死癌细胞而不是正常细胞，表明其在有效性方面的安全性。
