Parashar Manisha, Dhar Sanjoy Kumar, Kaur Jaspreet, Chauhan Arjun, Tamang Jeewan, Singh Gajendra Bahadur, Lyudmila Asyakina, Perveen Kahkashan, Khan Faheema, Bukhari Najat A, Mudgal Gaurav, Gururani Mayank Anand
University Institute of Biotechnology, Chandigarh University, Mohali 140413, Punjab,
Department of Biotechnology, Institute of Applied Sciences & Humanities, GLA University, Mathura 281406, Uttar Pradesh, India.
Plants (Basel). 2023 Aug 28;12(17):3081. doi: 10.3390/plants12173081.
Euphorbiaceae is a highly diverse family of plants ranging from trees to ground-dwelling minute plants. Many of these have multi-faceted attributes like ornamental, medicinal, industrial, and food-relevant values. In addition, they have been regarded as keystone resources for investigating plant-specific resilience mechanisms that grant them the dexterity to withstand harsh climates. In the present study, we isolated two co-culturable bacterial endophytes, EP1-AS and EP1-BM, from the stem internodal segments of the prostate spurge, , a plant member of the succulent family Euphorbiaceae. We characterized them using morphological, biochemical, and molecular techniques which revealed them as novel strains of Enterobacteriaceae, . Both the isolates significantly were qualified during the assaying of their plant growth promotion potentials. BM formed fast-growing swarms while AS showed growth as rounded colonies over nutrient agar. We validated the PGP effects of AS and BM isolates through in vitro and ex vitro seed-priming treatments with wheat and tomato, both of which resulted in significantly enhanced seed germination and morphometric and physiological plant growth profiles. In extended field trials, both AS and BM could remarkably also exhibit productive yields in wheat grain and tomato fruit harvests. This is probably the first-ever study in the context of PGPB endophytes in Euphorbia prostrata. We discuss our results in the context of promising agribiotechnology translations of the endophyte community associated with the otherwise neglected ground-dwelling spurges of Euphorbiaceae.
大戟科是一个高度多样化的植物家族，包括从树木到地面生长的微小植物。其中许多植物具有多方面的特性，如观赏、药用、工业和与食品相关的价值。此外，它们被视为研究植物特定适应机制的关键资源，这些机制使它们能够灵活地抵御恶劣气候。在本研究中，我们从肉质大戟科植物伏地大戟的茎节间分离出两种可共培养的细菌内生菌，EP1-AS和EP1-BM。我们使用形态学、生化和分子技术对它们进行了表征，结果表明它们是肠杆菌科的新菌株。在测定它们促进植物生长的潜力时，这两种分离物都表现出色。BM形成快速生长的菌落，而AS在营养琼脂上呈圆形菌落生长。我们通过对小麦和番茄进行体外和离体种子引发处理，验证了AS和BM分离物的植物生长促进作用，这两种处理均显著提高了种子发芽率以及植物的形态和生理生长指标。在扩展的田间试验中，AS和BM在小麦籽粒和番茄果实收获中也都能显著提高产量。这可能是关于伏地大戟中植物生长促进细菌内生菌的首次研究。我们在与大戟科中原本被忽视的地面生长大戟相关的内生菌群落有前景的农业生物技术转化背景下讨论了我们的结果。
