Jeon Byeong Jun, Park Jin-Soo, Hong Sung-Chul, Lee Eun Ha, Choi Jaeyoung, Kim Jeong Do
Smart Farm Research Center, Korea Institute of Science and Technology, Gangneung, Republic of Korea.
Natural Product Systems Biology Center, Korea Institute of Science and Technology, Gangneung, Republic of Korea.
Front Plant Sci. 2024 Nov 26;15:1484616. doi: 10.3389/fpls.2024.1484616. eCollection 2024.
Bacteria associated with plants play crucial roles in promoting plant growth and health by aiding in nutrient acquisition, including phosphorus. This study presents the isolation and genomic characterization of a potentially new bacterial strain, sp. JS-SCA-14, which exhibits significant plant growth-promoting effects through phosphorus solubilization. A comparative phylogenomic analysis of the complete genome of strain JS-SCA-14 and its closely related strains revealed a unique genomic profile, suggesting it could be a novel species. Genomic identity calculations indicated that JS-SCA-14 significantly deviates from strains belonging to closely related genera, such as , , , , and . A biochemical assay comparing JS-SCA-14 and a closely related strain, PFL01, showed differing patterns in carbon source utilization and enzyme activities. To assess the plant growth-promoting capabilities of strain JS-SCA-14, tests were conducted to evaluate its siderophore-producing and phosphate-solubilizing abilities. Seed germination assays demonstrated an improvement in germination, seedling length, and vigor compared to untreated controls. Notably, the phosphate-dissolving strain JS-SCA-14 led to a significant increase of 34.4% in fresh weight and 35.4% in dry weight of tomato plants compared to the negative control. These findings underscore the significant potential of strain JS-SCA-14 in solubilizing phosphate, thereby enhancing phosphorus availability in the rhizosphere and promoting plant growth and development. This study contributes to our understanding of plant-microbe interactions and suggests the potential application of strain JS-SCA-14 as a bioinoculant for sustainable agriculture and plant nutrient management strategies.
与植物相关的细菌通过协助植物获取包括磷在内的养分，在促进植物生长和健康方面发挥着关键作用。本研究展示了一种潜在的新细菌菌株——JS-SCA-14的分离及基因组特征，该菌株通过解磷表现出显著的植物促生长效应。对菌株JS-SCA-14及其密切相关菌株的全基因组进行的比较系统基因组分析揭示了独特的基因组图谱，表明它可能是一个新物种。基因组同一性计算表明，JS-SCA-14与属于密切相关属的菌株（如[此处原文缺失相关属名]）有显著差异。一项比较JS-SCA-14和密切相关菌株PFL01的生化分析显示，它们在碳源利用和酶活性方面存在不同模式。为评估菌株JS-SCA-14的植物促生长能力，进行了测试以评估其产铁载体和溶解磷酸盐的能力。种子发芽试验表明，与未处理的对照相比，发芽率、幼苗长度和活力均有所提高。值得注意的是，与阴性对照相比，解磷菌株JS-SCA-14使番茄植株的鲜重显著增加了34.4%，干重显著增加了35.4%。这些发现突出了菌株JS-SCA-14在溶解磷酸盐方面的巨大潜力，从而提高根际磷的有效性并促进植物生长发育。本研究有助于我们理解植物与微生物的相互作用，并表明菌株JS-SCA-14作为生物接种剂在可持续农业和植物养分管理策略中的潜在应用。
