Feijó Anderson da Rosa, Viana Vívian Ebeling, Balbinot Andrisa, Fipke Marcus Vinicius, Souza Gustavo Maia, do Amarante Luciano, Avila Luis Antonio de
Plant Physiology Graduate Program, Federal University of Pelotas, Pelotas 96160-000, Brazil.
Crop Protection Graduate Program, Federal University of Pelotas, Pelotas 96015-560, Brazil.
Plants (Basel). 2023 Aug 31;12(17):3133. doi: 10.3390/plants12173133.
Crop yields have been affected by many different biotic and abiotic factors. Generally, plants experience more than one stress during their life cycle, and plants can tolerate multiple stresses and develop cross-tolerance. The expected rise in atmospheric CO concentration ([CO]) can contribute to cross-tolerance. Priming is a strategy to increase yield or to maintain yield under stress conditions. Thus, our objective was to evaluate if priming the rice plants with water deficit during the vegetative stage can induce tolerance to heat stress at anthesis and to evaluate the contribution of [CO].
The experiment was arranged in a completely randomized design in a factorial arrangement. Factor A consisted of the following treatments: water deficit at four-leaf stage (no-stress, and drought stress), heat at anthesis (normal temperature, high temperature), and priming with water deficit at four-leaf stage and heat stress at anthesis; and Factor B was two [CO] treatments: [CO] = 400 ± 40 μmol mol and [CO] = 700 ± 40 μmol mol. We assessed the effect of the treatments on plant growth, yield, biochemical, and transcriptome alterations.
Although [CO] affected rice growth parameters, it did not affect the priming effect. Primed plants showed an increase in yield and number of panicles per plant. Primed plants showed upregulation of , , and . These results showed induced cross-tolerance.
Water deficit at the rice vegetative stage reduces the effect of heat stress at the reproductive stage. Water deficit at the vegetative stage can be used, after further testing in field conditions, to reduce the effect of heat stress during flowering in rice.
作物产量受到多种不同的生物和非生物因素影响。一般来说，植物在其生命周期中会经历多种胁迫，并且植物能够耐受多种胁迫并形成交叉耐受性。预计大气中二氧化碳浓度（[CO₂]）的升高可能有助于形成交叉耐受性。引发处理是一种在胁迫条件下提高产量或维持产量的策略。因此，我们的目标是评估在营养生长阶段对水稻进行水分亏缺引发处理是否能诱导其在花期对热胁迫产生耐受性，并评估[CO₂]的作用。
试验采用完全随机设计的析因排列。因素A包括以下处理：四叶期水分亏缺（无胁迫、干旱胁迫）、花期高温（常温、高温）以及四叶期水分亏缺引发处理和花期热胁迫；因素B为两种[CO₂]处理：[CO₂]=400±40 μmol/mol和[CO₂]=700±40 μmol/mol。我们评估了这些处理对植物生长、产量、生化和转录组变化的影响。
虽然[CO₂]影响水稻生长参数，但不影响引发处理的效果。引发处理的植株产量和单株穗数增加。引发处理的植株中[具体基因]、[具体基因]和[具体基因]上调。这些结果表明诱导了交叉耐受性。
水稻营养生长阶段的水分亏缺可降低生殖阶段热胁迫的影响。在田间条件下进一步测试后，营养生长阶段的水分亏缺可用于减轻水稻开花期热胁迫的影响。
