Poultry Production Dept., Faculty of Agriculture, Alexandria University, Alexandria, Egypt.
Animal and Poultry Production Dept., Faculty of Agriculture, Damanhour University, Damanhour, Egypt.
Anim Biotechnol. 2023 Dec;34(8):4060-4068. doi: 10.1080/10495398.2023.2255063. Epub 2023 Sep 8.
This study intended to assess the properties of in-ovo administration of Melittin (MLT) on hatchability, chick yield, hematology, immunological indices and relative organs weight of Alexandria chickens at hatch. A total of 600 eggs with an average weight of (45.12 g), were gathered and split into five groups: a non-injected group or negative control (NC), a saline injection group or positive control (PC), and three concentrations of MLT (5, 10 and 15 µg of MLT per egg, respectively). On day 18 of incubation, eggs from the injection groups were injected into the amniotic fluid from the large end with the in-ovo injection solutions (0.2 ml per egg). Results indicated that 10 µg MLT/egg positively affected the weight and yield of chicks. In addition, our findings indicated that the in-ovo administration with 10 or 15 µg MLT/egg was superior in most of the immunological indicators (spleen and bursa relative weights, immunoglobulins IgG and IgM, T cells and B cells). In conclusion, in order to improve the immune efficiency (early immune acceleration) of Alexandria chicks, which may contribute to offering a significant boost to their future performance, this study suggests injecting eggs with 5 or 10 µg MLT/egg.
本研究旨在评估在卵内注射蜂毒素（MLT）对孵化率、雏鸡产量、血液学、免疫指标和雏鸡相对器官重量的影响。共收集了 600 枚平均重量为（45.12 克）的鸡蛋，并将其分为五组：未注射组或阴性对照组（NC）、生理盐水注射组或阳性对照组（PC）以及三个浓度的 MLT（分别为 5、10 和 15μg/mlT 每枚鸡蛋）。在孵化的第 18 天，注射组的鸡蛋从大端的羊膜腔内用卵内注射溶液（每枚鸡蛋 0.2ml）注射。结果表明，10μg/mlT/枚鸡蛋可显著提高雏鸡的体重和产仔数。此外，我们的研究结果表明，10 或 15μg/mlT/枚鸡蛋的卵内注射在大多数免疫指标（脾脏和法氏囊相对重量、免疫球蛋白 IgG 和 IgM、T 细胞和 B 细胞）方面表现更好。总之，为了提高雏鸡的免疫效率（早期免疫加速），这可能对其未来的性能有显著提升，本研究建议注射 5 或 10μg/mlT/枚鸡蛋。
