Nanomaterials for Drug Delivery and Therapeutics (NDT-Lab), Centre for Nano and Material Science, Jain University, Jain Global Campus, Bengaluru, 562112, Karnataka, India.
Neural Developmental Biology Lab, Department of Life Science, NIT Rourkela, Odisha, Rourkela, 769008, India.
Environ Sci Pollut Res Int. 2024 Jul;31(34):46625-46640. doi: 10.1007/s11356-023-29160-x. Epub 2023 Sep 9.
The present study reports highly stable polymeric nanoparticles comprising curcumin and polyvinylpyrrolidone, and then conjugated with gold nanoparticles, resulting in C-PVP and C-PVP-Au, respectively. The synthesized conjugates C-PVP and C-PVP-Au were investigated for amyloid aggregation inhibition activity, antimicrobial activity, and wound healing applications. The anti-amyloidogenic capacity of nanoconjugates were studied for model protein, hen egg-white lysozyme (HEWL). The ThT binding assay, fibril size measurement, and electron microscopy results revealed that conjugates suppress fibrillogenesis in HEWL. The highest amyloid inhibition activity obtained against C-PVP and C-PVP-Au was 31 μg.mL and 30 μg.mL, respectively. The dissociation activity for amyloid aggregation was observed against Q-PVP and Q-PVP-Au at 29 μg.mL and 27 μg.mL, respectively. The antibacterial studies show significant efficacy against Escherichia coli (E. coli) in the presence of C-PVP and C-PVP-Au. The substantial antibacterial potential of C-PVP@PVA and C-PVP-Au@PVA membranes shows promising wound healing applications. The PVA membranes with nanoparticles promote the antibacterial activity and wound healing activity in the Drosophila model. C-PVP-Au@PVA membrane healed the wound faster than the C-PVP@PVA, and it can be used for better results in wound healing. Thus, C-PVP-Au and C-PVP have higher bioavailability and stability and can act as multifunctional therapeutic agents for amyloid-related diseases and as wound healing agents. Graphical abstract C-PVP, and C-PVP-Au conjugates for inhibition of HEWL aggregation, antibacterial and wound healing activity.
本研究报告了由姜黄素和聚乙烯吡咯烷酮组成的高度稳定的聚合物纳米粒子，然后与金纳米粒子缀合，分别得到 C-PVP 和 C-PVP-Au。合成的缀合物 C-PVP 和 C-PVP-Au 用于研究其对淀粉样蛋白聚集抑制活性、抗菌活性和伤口愈合应用。研究了纳米缀合物对模型蛋白鸡卵清溶菌酶（HEWL）的抗淀粉样蛋白形成能力。ThT 结合测定、纤维尺寸测量和电子显微镜结果表明，缀合物抑制了 HEWL 的纤维形成。对 C-PVP 和 C-PVP-Au 获得的最高淀粉样蛋白抑制活性分别为 31μg.mL 和 30μg.mL。观察到对 Q-PVP 和 Q-PVP-Au 的淀粉样蛋白聚集解离活性分别为 29μg.mL 和 27μg.mL。抗菌研究表明，C-PVP 和 C-PVP-Au 对大肠杆菌（E. coli）具有显著的疗效。C-PVP@PVA 和 C-PVP-Au@PVA 膜具有显著的抗菌潜力，显示出有前途的伤口愈合应用。载有纳米粒子的 PVA 膜可促进抗菌活性和伤口愈合活性在果蝇模型中。C-PVP-Au@PVA 膜比 C-PVP@PVA 更快地愈合伤口，可用于伤口愈合的更好结果。因此，C-PVP-Au 和 C-PVP 具有更高的生物利用度和稳定性，可作为与淀粉样蛋白相关疾病的多功能治疗剂和伤口愈合剂。
