College of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Hangzhou 310014, P. R. China.
Key Laboratory of Food Macromolecular Resources Processing Technology Research, (Zhejiang University of Technology), China National Light Industry, Hangzhou 310014, P.R. China.
Biomacromolecules. 2021 Feb 8;22(2):890-897. doi: 10.1021/acs.biomac.0c01599. Epub 2021 Jan 19.
Antibacterial agents with broad-spectrum antibacterial properties have always been in large demand. Lysozyme, a common and inexpensive protein, is widely used in food safety and biomedical applications for antibacterial purposes. However, many pathogens are lysozyme-resistant or insensitive. In this research, we investigated the antibacterial activities and mechanism of oligomers and amyloid fibrils formed from hen egg-white lysozyme (HEWL) against and . The HEWL fibrils showed significantly enhanced antibacterial activity against both lysozyme-resistant and lysozyme-insensitive . The HEWL oligomers, on the other hand, did not show an obvious improvement in antibacterial activity compared to native HEWL. Our results indicated that the fibrillation of HEWL can significantly enhance antibacterial activity against both and . The natural and inexpensive HEWL amyloid fibrils can be potentially applied to antimicrobial food packaging, animal feed, antibiotic replacement, etc.
具有广谱抗菌特性的抗菌剂一直以来都有很大的需求。溶菌酶是一种常见且廉价的蛋白质，广泛应用于食品安全和生物医药领域，具有抗菌作用。然而，许多病原体对溶菌酶具有抗性或不敏感。在这项研究中，我们研究了由鸡蛋清溶菌酶（HEWL）形成的低聚物和淀粉样纤维的抗菌活性和机制，以及它们对 和 的作用。HEWL 纤维对两种溶菌酶抗性的 和 都显示出显著增强的抗菌活性。相比之下，HEWL 低聚物的抗菌活性并没有比天然 HEWL 明显提高。我们的结果表明，HEWL 的纤维化可以显著增强对 和 的抗菌活性。天然且廉价的 HEWL 淀粉样纤维可潜在应用于抗菌食品包装、动物饲料、抗生素替代等领域。
