用于荧光激活细胞分选嗅感觉神经元的小鼠嗅黏膜分离。
Dissociation of Mouse Olfactory Mucosae for Fluorescence-Activated Cell Sorting of Olfactory Sensory Neurons.
机构信息
Department of Physiology, University of Kentucky, Lexington, KY, USA.
Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY, USA.
出版信息
Methods Mol Biol. 2023;2710:111-120. doi: 10.1007/978-1-0716-3425-7_9.
An increasing diversity of techniques investigating the biology of specific cell types and individual cells have elevated the importance of dissociation of viable cells from living tissues. Here we describe a method for the dissociation of single cells from samples of adult mouse olfactory mucosae, with an emphasis on maximizing yield of viable single cells from fluorescence-activated cell sorting. Yields are typically in the range of 80,000-150,000 viable cells per adult mouse.
越来越多的技术被用于研究特定细胞类型和单个细胞的生物学特性，这使得从活体组织中分离有活力的细胞变得尤为重要。本文描述了一种从成年小鼠嗅黏膜样本中分离单细胞的方法，重点是从荧光激活细胞分选（FACS）中最大限度地提高有活力的单细胞产量。每个成年小鼠的产量通常在 80,000-150,000 个有活力的细胞之间。
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