利用 Slide-SeqV2 鉴定和定位小鼠嗅球中的细胞类型。
Identification and Localization of Cell Types in the Mouse Olfactory Bulb Using Slide-SeqV2.
机构信息
Stowers Institute for Medical Research, Kansas City, MO, USA.
Department of Cell Biology and Physiology, University of Kansas Medical Center, Kansas City, KS, USA.
出版信息
Methods Mol Biol. 2023;2710:171-183. doi: 10.1007/978-1-0716-3425-7_13.
Spatial transcriptomics maps RNA molecules to the location in a tissue where they are expressed. Here we document the use of Slide-SeqV2 to visualize gene expression in the mouse olfactory bulb (OB). This approach relies on spatially identified beads to locate and quantify individual transcripts. The expression profiles associated with the beads are used to identify and localize individual cell types in an unbiased manner. We demonstrate the various cell types and subtypes with distinct spatial locations in the olfactory bulb that are identified using Slide-SeqV2.
空间转录组学将 RNA 分子映射到其在组织中表达的位置。本文记录了使用 Slide-SeqV2 可视化小鼠嗅球（OB）中基因表达的情况。该方法依赖于空间定位的珠子来定位和定量单个转录本。与珠子相关的表达谱可用于以无偏倚的方式识别和定位单个细胞类型。我们利用 Slide-SeqV2 展示了在嗅球中具有不同空间位置的各种细胞类型和亚型。
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