Division of Crop Protection, ICAR-Indian Institute of Vegetable Research, Varanasi, 221305, India.
Division of Crop Protection, ICAR-Indian Institute of Vegetable Research, Varanasi, 221305, India.
Virology. 2023 Oct;587:109876. doi: 10.1016/j.virol.2023.109876. Epub 2023 Sep 2.
Totally 102 symptomatic samples of cucurbitaceous vegetables showing yellowing were collected from fields of Uttar Pradesh and screened by RT-PCR assay for the presence of Crinivirus and Polerovirus. Among them, Crinivirus (16%) and Polerovirus (23%) were tested positive with the universal primer pairs. Based on the sequence analysis of amplified product, two Crinivirus (Cucurbit chlorotic yellows virus - CCYV and Cucurbit yellow stunting disorder virus - CYSDV) and two Polerovirus (Cucurbit aphid-borne yellows virus - CABYV and Luffa aphid-borne yellows virus - LABYV) species were characterized. Phylogenetic analysis revealed less genetic distance among the Indian isolates of CCYV, CYSDV and LABYV whereas CABYV closely related to Chinese isolates. To the best of our knowledge, this study documents infection of CCYV on cucumber, round melon and muskmelon; CYSDV on satputia and sponge gourd; CABYV on ivy gourd; and LABYV on ridge gourd, satputia and muskmelon for the first time in India.
共采集了来自北方邦田间表现为黄化的 102 个葫芦科蔬菜有症状样本，通过 RT-PCR 检测法对其是否存在藜芦科病毒和马铃薯 Y 病毒进行了筛查。其中，16%的样本检测到藜芦科病毒（Crinivirus）呈阳性，23%的样本检测到马铃薯 Y 病毒（Polerovirus）呈阳性，所用的是通用引物对。根据扩增产物的序列分析，鉴定出两种藜芦科病毒（瓜类褪绿黄化病毒 - CCYV 和瓜类黄矮化病毒 - CYSDV）和两种马铃薯 Y 病毒（瓜蚜传黄花叶病毒 - CABYV 和丝瓜蚜传黄花叶病毒 - LABYV）。系统发育分析表明，印度分离株的 CCYV、CYSDV 和 LABYV 之间遗传距离较小，而 CABYV 与中国分离株关系密切。据我们所知，本研究首次在印度报道了 CCYV 对黄瓜、圆瓜和甜瓜的感染；CYSDV 对 satputia 和丝瓜的感染；CABYV 对 ivy gourd 的感染；以及 LABYV 对 ridged gourd、satputia 和甜瓜的感染。
