Stowell C P, Scanlin T F, Glick M C
Carbohydr Res. 1986 Aug 15;151:279-92. doi: 10.1016/s0008-6215(00)90348-3.
Fibronectins isolated from different species and tissue sources are glycosylated differently. We report here a characterization of the glycopeptides of fibronectin isolated from the culture medium of skin fibroblasts from patients with cystic fibrosis together with age-, race-, and sex-matched control subjects. The characterization of this fibronectin is of special interest because it is derived from: a non-fetal, cellular source; eight different individuals; and cystic fibrosis and control individuals. The fibronectin glycopeptides were purified by gel-permeation chromatography and Con A-Sepharose and were analyzed by anion-exchange chromatography and affinity columns of immobilized 5-hydroxytryptamine and lectins. One half of the glycopeptides of skin fibroblast fibronectin were shown to contain biantennary oligosaccharides which were core-fucosylated and partially sialylated. Although the remaining half was a complex mixture of glycopeptides, there was remarkably little inter-individual variation. No difference between cystic fibrosis and control subjects was discernible by the techniques employed here. Unlike the biantennary glycopeptides of human plasma fibronectin, those from skin fibroblast fibronectin were core-fucosylated and less highly sialylated. However, compared to human cellular fibronectin glycopeptides from fetal sources, those from skin fibroblast fibronectin were both more highly fucosylated and sialylated.
从不同物种和组织来源分离出的纤连蛋白糖基化方式不同。我们在此报告从囊性纤维化患者及年龄、种族和性别匹配的对照受试者的皮肤成纤维细胞培养基中分离出的纤连蛋白糖肽的特性。这种纤连蛋白的特性特别引人关注，因为它源自：非胎儿的细胞来源；八个不同个体；以及囊性纤维化患者和对照个体。纤连蛋白糖肽通过凝胶渗透色谱法和伴刀豆球蛋白A-琼脂糖进行纯化，并通过阴离子交换色谱法以及固定化5-羟色胺和凝集素的亲和柱进行分析。结果显示，皮肤成纤维细胞纤连蛋白的一半糖肽含有双天线寡糖，这些寡糖进行了核心岩藻糖基化且部分唾液酸化。尽管其余一半是糖肽的复杂混合物，但个体间差异非常小。通过此处采用的技术，未发现囊性纤维化患者和对照受试者之间存在差异。与人类血浆纤连蛋白的双天线糖肽不同，皮肤成纤维细胞纤连蛋白的双天线糖肽进行了核心岩藻糖基化且唾液酸化程度较低。然而，与来自胎儿来源的人类细胞纤连蛋白糖肽相比，皮肤成纤维细胞纤连蛋白的糖肽岩藻糖基化和唾液酸化程度都更高。
