载脂蛋白 C3 在卵母细胞中表达，其表达增加与 PCOS 的进展有关。

ApoC3 is expressed in oocytes and increased expression is associated with PCOS progression.

机构信息

Guangdong Women and Children Hospital, Guangzhou, 511442, China.

Guangzhou Medical University, Guangzhou, 511436, China.

出版信息

J Ovarian Res. 2023 Sep 9;16(1):188. doi: 10.1186/s13048-023-01263-6.

DOI:10.1186/s13048-023-01263-6

PMID:37689737

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10493025/

Abstract

BACKGROUND

Polycystic ovary syndrome (PCOS) is a lifelong metabolic disorder and the most common cause of anovulatory infertility affecting women in reproductive age. Our recent study reported that apolipoprotein C3 (ApoC3) could be a potential diagnostic serum marker for metabolism disturbance in PCOS patients, but whether it is present in the ovaries and what role it plays has not yet been described.

OBJECTIVE

Aimed to investigate ApoC3 expression in ovary of PCOS, and to discuss its potential role in PCOS progression.

METHODS

ApoC3 expression in ovarian tissue samples from 12 PCOS patients along with 12 healthy controls were measured via immunohistochemistry (IHC). Also, the level of ApoC3 in follicular fluid from 14 patients diagnosed with PCOS and 13 control subjects were detected by ELISA. The expression and location of ApoC3 in ovaries of PCOS mice were tested weekly for three consecutive weeks during PCOS formation using real time PCR, Western Blot, IHC and immunofluorescence. The relation of ApoC3 and sex hormones was analyzed in mouse plasma. Additionally, the dynamic changes of ApoC3 level in ovaries of healthy mice during postnatal development was also investigated.

RESULTS

ApoC3 levels in ovarian tissue and follicular fluid were significantly higher in PCOS patients than in controls (33.87 ± 4.11 vs. 27.71 ± 3.65, P < 0.01; 0.87 ± 0.09 vs. 0.51 ± 0.32 ng/mL, P < 0.05), respectively. In ovary, ApoC3 was found to be located in the cytoplasm of oocyte, and its expression gradually increased with PCOS progression (P < 0.05). Furthermore, correlation analysis showed that plasma ApoC3 level was closely associated with luteinizing hormone (r = 0.709, P = 0.001), testosterone (r = 0.627, P = 0.005) and anti-mullerian hormone (r = 0.680, P = 0.002) in PCOS mice. In addition, ApoC3 level in oocyte was physiologically increased and peaked on postnatal age 21 (P21), then decreased following P21 in healthy mice.

CONCLUSIONS

We identified ApoC3 expression in oocyte. It may be involved in PCOS progression and possibly participate in the regulation of oocyte development.

摘要

背景

多囊卵巢综合征（PCOS）是一种终身代谢紊乱疾病，也是导致育龄期妇女排卵障碍性不孕的最常见原因。我们最近的研究报告表明，载脂蛋白 C3（ApoC3）可能是 PCOS 患者代谢紊乱的潜在诊断血清标志物，但它是否存在于卵巢中以及发挥什么作用尚未描述。

目的

旨在研究 ApoC3 在 PCOS 卵巢中的表达，并探讨其在 PCOS 进展中的潜在作用。

方法

通过免疫组织化学（IHC）检测 12 例 PCOS 患者和 12 例健康对照者卵巢组织样本中的 ApoC3 表达。此外，通过 ELISA 检测 14 例 PCOS 患者和 13 例对照组患者卵泡液中的 ApoC3 水平。使用实时 PCR、Western Blot、IHC 和免疫荧光法，在 PCOS 形成的连续 3 周内每周检测 PCOS 小鼠卵巢中 ApoC3 的表达和位置。分析了 PCOS 小鼠血浆中 ApoC3 与性激素的关系。此外，还研究了健康小鼠在产后发育过程中卵巢中 ApoC3 水平的动态变化。

结果

PCOS 患者卵巢组织和卵泡液中的 ApoC3 水平明显高于对照组（33.87±4.11 与 27.71±3.65，P<0.01；0.87±0.09 与 0.51±0.32ng/ml，P<0.05）。在卵巢中，发现 ApoC3 位于卵母细胞的细胞质中，其表达随着 PCOS 的进展而逐渐增加（P<0.05）。此外，相关性分析表明，PCOS 小鼠血浆 ApoC3 水平与黄体生成素（r=0.709，P=0.001）、睾酮（r=0.627，P=0.005）和抗苗勒管激素（r=0.680，P=0.002）密切相关。此外，在健康小鼠中，卵母细胞中的 ApoC3 水平在出生后第 21 天（P21）生理性增加并达到峰值，然后在 P21 后下降。