Mechanical Engineering Faculty (FEM), State University of Campinas (UNICAMP), Campinas, SP, Brazil; Laboratory of Biomaterials, Faculty of Medical Sciences and Health (FCMS), Pontifical Catholic University of São Paulo (PUC-SP), Sorocaba, SP, Brazil.
Laboratory of Biomaterials, Faculty of Medical Sciences and Health (FCMS), Pontifical Catholic University of São Paulo (PUC-SP), Sorocaba, SP, Brazil; Postgraduate Program in Materials Sciences (PPGCM), Federal University of São Carlos (UFSCar), Sorocaba, SP, Brazil.
Int J Biol Macromol. 2023 Dec 31;253(Pt 2):126782. doi: 10.1016/j.ijbiomac.2023.126782. Epub 2023 Sep 9.
Latex is a colloidal suspension derived from the Hevea brasiliensis tree, derived from natural rubber, poly(isoprene), and assorted constituents including proteins and phospholipids. These constituents are inherent to both natural rubber and latex serum. This investigation was undertaken to examine the impact of the deproteinization process on chemical and biological dynamics of natural rubber latex. Natural Rubber (NR) extracted from the pure latex (LNCP) was obtained through centrifugation, followed by six rounds of solvent purification (LP6). The structure was characterized using Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), swelling test, surface zeta potential (ζ), scanning electron microscopy (SEM) and in vitro assay. The results revealed that the LP6 group presented decreased swelling kinetics, reduced cell adhesion and proliferation, and a smoother surface with decreased negative surface charge. Conversely, the LNCP group shown accelerated swelling, heightened adhesion and cellular growth, and a more negatively charged and rougher surface. As such, the attributes of latex serum and proteins have potential usage across numerous biomedical applications.
胶乳是一种源自巴西橡胶树的胶体悬浮液，源自天然橡胶，聚异戊二烯和各种成分，包括蛋白质和磷脂。这些成分是天然橡胶和乳胶血清所固有的。这项研究旨在研究脱蛋白过程对天然橡胶胶乳的化学和生物学动态的影响。通过离心从纯胶乳（LNCP）中提取天然橡胶（NR），然后通过六轮溶剂纯化（LP6）。使用傅里叶变换红外光谱（FTIR），热重分析（TGA），差示扫描量热法（DSC），溶胀试验，表面zeta 电位（ζ），扫描电子显微镜（SEM）和体外测定来表征结构。结果表明，LP6 组表现出较慢的溶胀动力学，减少了细胞粘附和增殖，表面更光滑，表面负电荷减少。相反，LNCP 组显示出加速的溶胀，更高的粘附和细胞生长，以及更负的电荷和更粗糙的表面。因此，乳胶血清和蛋白质的特性具有在多种生物医学应用中的潜在用途。
