Silva Franciéle M, Silva Jaqueline R, Rodrigues Wellington, Sousa Breno A S M, Gomes Thamis F S, Rosa Mario F F, Rosa Suélia S R F, Carneiro Marcella L B
Institute of Biological Sciences, University of Brasília, Brasília 73345-010, DF, Brazil.
Faculty of Gama, University of Brasília, Brasília 73345-010, DF, Brazil.
Pharmaceuticals (Basel). 2025 Jul 20;18(7):1067. doi: 10.3390/ph18071067.
Wound healing in diabetic individuals is a prolonged process, often complicated by infections and impaired tissue regeneration. Innovative strategies combining natural bioactive compounds are needed to enhance repair. This study reports the development and characterization of natural latex-based biomembranes (NLBs) incorporated with liposome-encapsulated curcumin and papain. The therapeutic efficacy of these composite dressings, in combination with red light-emitting diode (LED) phototherapy, was evaluated in a diabetic rat model. NLBs were produced by blending natural latex with multilamellar liposomes containing either curcumin, papain, or both. In vivo wound healing was assessed by applying the biomembranes to the dorsal lesions and administering red LED irradiation (650 ± 20 nm, 10 min every 48 h) over 11 days. Fourier transform infrared spectroscopy (FTIR) confirmed that the integration of liposomes did not induce significant chemical alterations to the latex matrix. The treated diabetic rats exhibited enhanced wound contraction, with the curcumin and papain groups demonstrating up to 99% and 95% healing, respectively. Plasma fructosamine levels were significantly reduced ( < 0.05), indicating improved glycemic control. Combining NLBs with bioactive-loaded liposomes and phototherapy accelerated wound healing in diabetic rats. This multifunctional platform shows promise for the treatment of chronic wounds in diabetic patients.
糖尿病患者的伤口愈合过程漫长，常因感染和组织再生受损而复杂化。需要创新策略来结合天然生物活性化合物以促进修复。本研究报告了负载脂质体包裹姜黄素和木瓜蛋白酶的天然乳胶基生物膜（NLBs）的开发与特性。在糖尿病大鼠模型中评估了这些复合敷料与红光发光二极管（LED）光疗相结合的治疗效果。通过将天然乳胶与含有姜黄素、木瓜蛋白酶或两者的多层脂质体混合来制备NLBs。通过将生物膜应用于背部伤口并在11天内进行红色LED照射（650±20nm，每48小时10分钟）来评估体内伤口愈合情况。傅里叶变换红外光谱（FTIR）证实脂质体的整合未对乳胶基质引起显著的化学变化。治疗的糖尿病大鼠伤口收缩增强，姜黄素组和木瓜蛋白酶组分别显示高达99%和95%的愈合率。血浆果糖胺水平显著降低（<0.05），表明血糖控制得到改善。将NLBs与负载生物活性的脂质体和光疗相结合可加速糖尿病大鼠的伤口愈合。这个多功能平台有望用于治疗糖尿病患者的慢性伤口。
