Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, Campo Boario, 64100 Teramo, Italy.
Service de Microbiologie, Immunologie et Pathologie Générale, École Nationale de Médecine Vétérinaire de Sidi Thabet, IRESA, Université de la Manouba, Tunisia.
J Virol Methods. 2023 Nov;321:114808. doi: 10.1016/j.jviromet.2023.114808. Epub 2023 Sep 9.
Epizootic hemorrhagic disease virus (EHDV) is a Culicoides-transmitted virus circulating in multiple serotypes. It has become a concern in the European Union as a novel strain of the serotype 8 (EHDV-8) of clear Northern African origin, has been recently discovered in symptomatic cattle in Italy (islands of Sardinia and Sicily), Spain, and Portugal. Current molecular typing methods targeting the S2 nucleotide sequences -coding for the outermost protein of the virion VP2- are not able to detect the novel emerging EHDV-8 strain as they enrolled the S2 sequence of the unique EHDV-8 reference strain isolated in Australia in 1982. Thus, in this study, we developed and validated a novel typing assay for the detection and quantitation of the novel EHDV-8 RNA from field samples, including blood of ruminants and insects. This molecular tool will certainly support EHDV-8 surveillance and control.
虫媒病毒（EHDV）是一种由库蠓传播的病毒，存在多种血清型。由于一种新型的 8 型血清（EHDV-8）源自明显的北非地区，因此在欧盟引起了关注，该病毒最近在意大利（撒丁岛和西西里岛）、西班牙和葡萄牙出现症状的牛群中被发现。目前针对 S2 核苷酸序列（编码病毒粒子最外层蛋白 VP2）的分子分型方法无法检测到新型的 EHDV-8 毒株，因为它们使用的是 1982 年在澳大利亚分离到的独特 EHDV-8 参考毒株的 S2 序列。因此，在这项研究中，我们开发并验证了一种新的检测方法，用于从野外样本（包括反刍动物和昆虫的血液）中检测和定量新型 EHDV-8 RNA。这种分子工具肯定会支持 EHDV-8 的监测和控制。
