Jing MingFei, Yang Wen, Ding Xiuying, Rao Lihua, Zhang Quanxiang, Zhu Jinyong
School of Marine Science, Ningbo University, Ningbo 315800, China.
Hangzhou West Lake Administration, Hangzhou 310002, China.
Sci Total Environ. 2023 Dec 10;903:166943. doi: 10.1016/j.scitotenv.2023.166943. Epub 2023 Sep 9.
With the development of global tourism, tourist boats, a significant form of anthropogenic disturbance, are having an increasingly serious impact on the structure and function of aquatic ecosystems. In this study, the effects of different intensities of tourist boat activities on the microbial communities of West lake, were investigated by high-throughput sequencing. The results showed significant differences in the composition of bacterioplankton and microeukaryotic communities between the high-intensity boat activity (HIBA) area and low-intensity boat activity (LIBA) area. Variation partitioning analysis showed that environmental factors contributed the most to microbial community variation, and the effect of boat activities on microbial communities mainly occurred through coupling with environmental factors. The contribution of boat activity to microbial community changes occupies the second place, the first being environmental factors. Co-occurrence network analyses showed that microbial communities in the HIBA area had more nodes and edges, higher connectivity and lower modularity than in the LIBA area, suggesting a more complex and stable network. Networks of associations between potential keystone taxa and environmental factors reveal the way in which boat activity affects microbial communities. The bacterial community responded strongly to environmental factors associated with boat activities, whereas the microeukaryotic community was more likely to be regulated by interspecific interactions. This also suggests that when faced with disturbances from the boat activity, microeukaryotes might exert a stronger direct resistance effect compared to bacterioplankton. These findings imply that bacterioplankton and microeukaryotes demonstrate distinct response patterns in the presence of disturbance caused by boat activity. Our research expand our understanding of the effects of boat activities on aquatic ecosystems and provide further insights into the assessment of anthropogenic disturbances in aquatic ecosystems.
随着全球旅游业的发展，游船作为一种重要的人为干扰形式，对水生生态系统的结构和功能产生着日益严重的影响。在本研究中，通过高通量测序研究了不同强度游船活动对西湖微生物群落的影响。结果表明，高强度游船活动（HIBA）区域和低强度游船活动（LIBA）区域的浮游细菌和微型真核生物群落组成存在显著差异。变异分解分析表明，环境因素对微生物群落变异的贡献最大，游船活动对微生物群落的影响主要通过与环境因素的耦合发生。游船活动对微生物群落变化的贡献位居第二，第一是环境因素。共现网络分析表明，HIBA区域的微生物群落比LIBA区域具有更多的节点和边、更高的连通性和更低的模块性，表明网络更复杂和稳定。潜在关键类群与环境因素之间的关联网络揭示了游船活动影响微生物群落的方式。细菌群落对与游船活动相关的环境因素反应强烈，而微型真核生物群落更可能受到种间相互作用的调节。这也表明，面对游船活动的干扰时，微型真核生物可能比浮游细菌表现出更强的直接抵抗作用。这些发现意味着浮游细菌和微型真核生物在游船活动引起的干扰存在时表现出不同的响应模式。我们的研究扩展了我们对游船活动对水生生态系统影响的理解，并为评估水生生态系统中的人为干扰提供了进一步的见解。
