Yang Jing, Liang Yuan, Li Xiang, Zhang Yongpeng, Qian Long, Ke Yonggang, Zhang Cheng
School of Computer Science, Key Lab of High Confidence Software Technologies, Peking University, Beijing, 100871, China.
School of Control and Computer Engineering, North China Electric Power University, Beijing, 102206, China.
Angew Chem Int Ed Engl. 2023 Oct 23;62(43):e202308797. doi: 10.1002/anie.202308797. Epub 2023 Sep 19.
Programmable assembly of gold nanoparticle superstructures with precise spatial arrangement has drawn much attention for their unique characteristics in plasmonics and biomedicine. Bio-inspired methods have already provided programmable, molecular approaches to direct AuNP assemblies using biopolymers. The existing methods, however, predominantly use DNA as scaffolds to directly guide the AuNP interactions to produce intended superstructures. New paradigms for regulating AuNP assembly will greatly enrich the toolbox for DNA-directed AuNP manipulation and fabrication. Here, we developed a strategy of using a spatially programmable enzymatic nanorobot arm to modulate anisotropic DNA surface modifications and assembly of AuNPs. Through spatial controls of the proximity of the reactants, the locations of the modifications were precisely regulated. We demonstrated the control of the modifications on a single 15 nm AuNP, as well as on a rectangular DNA origami platform, to direct unique anisotropic AuNP assemblies. This method adds an alternative enzymatic manipulation to DNA-directed AuNP superstructure assembly.
具有精确空间排列的金纳米粒子超结构的可编程组装因其在等离子体学和生物医学中的独特特性而备受关注。受生物启发的方法已经提供了使用生物聚合物来指导金纳米粒子组装的可编程分子方法。然而，现有方法主要使用DNA作为支架来直接引导金纳米粒子相互作用以产生预期的超结构。调节金纳米粒子组装的新范例将极大地丰富用于DNA定向金纳米粒子操纵和制造的工具箱。在这里，我们开发了一种使用空间可编程酶促纳米机器人臂来调节金纳米粒子的各向异性DNA表面修饰和组装的策略。通过对反应物接近度的空间控制，修饰的位置得到了精确调节。我们展示了对单个15纳米金纳米粒子以及矩形DNA折纸平台上的修饰进行控制，以引导独特的各向异性金纳米粒子组装。该方法为DNA定向金纳米粒子超结构组装增加了一种替代的酶促操纵方法。
