从植物病原菌胶孢炭疽菌中分离到一种新型呼肠孤病毒的特性。
Characterization of a novel hypovirus isolated from the phytopathogenic fungus Colletotrichum camelliae.
机构信息
National Key Laboratory of Agricultural Microbiology, Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Institute of Plant Protection, Guangxi Academy of Agricultural Science, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, 530007, Guangxi, China.
出版信息
Arch Virol. 2023 Sep 10;168(10):250. doi: 10.1007/s00705-023-05878-4.
Some members of genus Colletotrichum are important plant pathogens. Here, we report a novel positive single-stranded RNA virus, Colletotrichum camelliae hypovirus 1 (CcHV1), from strain GXNN11-2 of Colletotrichum camelliae. The complete genome of CcHV1 is 9907 nucleotides (nt) in length and contains a single large open reading frame (ORF) from nt 352 to 9006. This ORF encodes a polyprotein with four conserved domains, namely UDP-glycosyltransferase, RNA-dependent RNA polymerase (RdRp), peptidase, and DEAD-like helicase. The CcHV1 polyprotein shares the highest similarity with Fusarium concentricum hypovirus 1. Phylogenetic analysis indicated that CcHV1 clustered with members of the genus Betahypovirus within the family Hypoviridae. This is the first report of a hypovirus in a member of the genus Colletotrichum.
一些炭疽菌属成员是重要的植物病原体。在这里，我们报道了一种新型的正单链 RNA 病毒，即来自炭疽菌菌株 GXNN11-2 的茶炭疽菌呼肠孤病毒 1（CcHV1）。CcHV1 的完整基因组长 9907 个核苷酸（nt），包含一个从 nt 352 到 9006 的单一长开放阅读框（ORF）。该 ORF 编码一个具有四个保守结构域的多蛋白，即 UDP-糖基转移酶、RNA 依赖性 RNA 聚合酶（RdRp）、肽酶和 DEAD 样解旋酶。CcHV1 多蛋白与尖孢镰刀菌呼肠孤病毒 1 具有最高的相似性。系统发育分析表明，CcHV1 与呼肠孤病毒科贝塔呼肠孤病毒属的成员聚为一簇。这是首次在炭疽菌属成员中报道呼肠孤病毒。
Zotero 插件