Gold D V
J Natl Cancer Inst. 1979 Jul;63(1):105-9.
We purified a high-molecular-weight colon mucoprotein antigen (CMA) from normal F344 rat colon and from a transplantable dimethylhydrazine-induced colon carcinoma. Chemical analysis of the mucins showed similar amino acid and carbohydrate compositions. This finding was in contrast to the major differences that occurred in the composition of human CMA as a consequence of neoplasia. Immunohistochemical techniques were used in an examination of organ and tumor specificities. A New Zealand White rabbit antiserum against purified normal rat CMA, appropriately absorbed, detected a normal colon-specific determinant(s). The organ-specific determinant was lost as a consequence of neoplasia. However, a new tumor-specific determinant was then detected. CMA may have a potential role as an organ-specific marker of the neoplastic process.
我们从正常的F344大鼠结肠以及可移植的二甲基肼诱导的结肠癌中纯化出一种高分子量结肠粘蛋白抗原（CMA）。对这些粘蛋白的化学分析显示，其氨基酸和碳水化合物组成相似。这一发现与人类CMA因肿瘤形成而在组成上出现的主要差异形成对比。采用免疫组织化学技术检查器官和肿瘤特异性。一种针对纯化的正常大鼠CMA的新西兰白兔抗血清，经过适当吸收后，检测到一种正常结肠特异性决定簇。这种器官特异性决定簇因肿瘤形成而丧失。然而，随后检测到一种新的肿瘤特异性决定簇。CMA可能作为肿瘤形成过程的器官特异性标志物具有潜在作用。
