Gold D V, Miller F
Tissue Antigens. 1978 Apr;11(4):362-71. doi: 10.1111/j.1399-0039.1978.tb01269.x.
The tissue specificity of colonic mucoprotein antigen (CMA) was examined to determine whether the antigen might have a role as a tissue marker. The immunofluorescent technique, using a rabbit anti-CMA antiserum, was employed to examine a wide range of tissues. Gastrointestinal mucosae as well as non-gastrointestinal mucin producing tissues were positive; however, non-gastrointestinal reactivity could be eliminated by the appropriate absorptions. Mucoproteins were purified separately from each gastrointestinal anatomic region and used to absorb the antiserum. This analysis demonstrated the existence of a family of mucoproteins with a common gastrointestinal specific determinant(s). Mucoproteins from neighboring regions, as seen by this antiserum, showed a greater structural similarity than did mucoproteins from distant regions. Absorption of the antiserum with ileal or cecal mucoprotein allowed the detection of a colon specific determinant present on CMA.
检测结肠粘蛋白抗原（CMA）的组织特异性，以确定该抗原是否可能作为一种组织标志物。采用兔抗CMA抗血清的免疫荧光技术检测了多种组织。胃肠道黏膜以及非胃肠道产生粘蛋白的组织均呈阳性；然而，通过适当的吸收可消除非胃肠道反应性。从每个胃肠道解剖区域分别纯化粘蛋白，并用于吸收抗血清。该分析证明存在具有共同胃肠道特异性决定簇的粘蛋白家族。用该抗血清观察到，相邻区域的粘蛋白比远距离区域的粘蛋白显示出更大的结构相似性。用回肠或盲肠粘蛋白吸收抗血清可检测到CMA上存在的结肠特异性决定簇。
